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D0550

3,5-Dinitrosalicylic acid

used in colorimetric determination of reducing sugars

Synonym(s):

3,5-Dinitro-2-hydroxybenzoic acid, DNS

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About This Item

Linear Formula:
(O2N)2C6H2-2-(OH)CO2H
CAS Number:
609-99-4
Molecular Weight:
228.12
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24893243
EC Number:
210-204-3
Beilstein/REAXYS Number:
2220661
MDL number:
MFCD00007104

Key Documents

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Product Name

3,5-Dinitrosalicylic acid, used in colorimetric determination of reducing sugars

InChI key

LWFUFLREGJMOIZ-UHFFFAOYSA-N

InChI

1S/C7H4N2O7/c10-6-4(7(11)12)1-3(8(13)14)2-5(6)9(15)16/h1-2,10H,(H,11,12)

SMILES string

OC(=O)c1cc(cc(c1O)[N+]([O-])=O)[N+]([O-])=O

assay

≥98% (HPLC and titration)

form

powder

mp

168-172 °C (lit.)

solubility

H2O: 50 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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1 of 4

This Item
128848D124001136883
3,5-Dinitrosalicylic acid used in colorimetric determination of reducing sugars

Sigma-Aldrich

D0550

3,5-Dinitrosalicylic acid

3,5-Dinitrosalicylic acid 98%

Sigma-Aldrich

128848

3,5-Dinitrosalicylic acid

3,5-Diiodosalicylic acid 99%

Sigma-Aldrich

D124001

3,5-Diiodosalicylic acid

4-Methyl-3,5-dinitrobenzoic acid 98%

Sigma-Aldrich

136883

4-Methyl-3,5-dinitrobenzoic acid

assay

≥98% (HPLC and titration)

assay

98%

assay

99%

assay

98%

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

100

solubility

H2O: 50 mg/mL

solubility

-

solubility

-

solubility

methanol: soluble 250 mg/10 mL, clear, faintly yellow to yellow

storage temp.

room temp

storage temp.

-

storage temp.

-

storage temp.

-

form

powder

form

powder

form

powder

form

solid

mp

168-172 °C (lit.)

mp

168-172 °C (lit.)

mp

220-230 °C (dec.) (lit.)

mp

155-158 °C (lit.)

Application

3,5-Dinitrosalicylic acid (DNS) has been used to stop the reaction in in vitro α-amylase inhibition study.[1] It has also been used for the quantitation of enzymatically released reducing sugars.[2]

Biochem/physiol Actions

3,5-Dinitrosalicylic acid (DNS) is used in colorimetric determination of reducing sugars[3][4] and to analyze glycosidase (glycoside hydrolase) activity by quantitation of enzymatically released reducing sugar. The dinitrosalicylic acid method has been compared to the Nelson-Somogi colorimetric method.[5][6]

pictograms

CorrosionExclamation mark
GHS05,GHS07

signalword

Danger

hcodes

H302,H318

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1

Storage Class

4.1A - Other explosive hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
MKCZ1057
MKCX9507
MKCX2724
MKCW6704
MKCW4641

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Studies on the ecology of actinomycetes in an agricultural soil amended with organic residues: II. Assessment of enzymatic activities of Actinomycetales isolates.
Mokni-Tlili S, et al.
World Journal of Microbiology & Biotechnology, 27, 2251-2259 (2011)
Comparison of the 3,5-dinitrosalicylicacid and Nelson-Somogyi methods of assaying for reducing sugars and determining cellulase activity.
Breuil, C. and Saddler, J.N.
Enzyme and Microbial Technology, 7, 327-332 (1985)
Use of dinitrosalicylic acid reagent for determination of reducing sugar.
Miller, G.L.
Analytical Chemistry, 31, 426-428 (1959)
Reducing value methods for maltodextrins. I. Chain-length dependence of alkaline 3,5-dinitrosalicylate and chain-length independence of alkaline copper.
J F Robyt et al.
Analytical biochemistry, 45(2), 510-516 (1972-02-01)
Rammohan Subramanian et al.
Acta biochimica Polonica, 55(2), 391-398 (2008-05-31)
There has been an enormous interest in the development of alternative medicines for type 2 diabetes, specifically screening for phytochemicals with the ability to delay or prevent glucose absorption. The goal of the present study was to provide in vitro
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Protocols

Enzymatic Assay of α-Amylase (EC 3.2.1.1)

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

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