Skip to Content
Merck
All Photos(1)

Documents

E3789

Sigma-Aldrich

Amphotericin B – Low Endotoxin

Less than 5 EU/mg, suitable for cell culture

Sign Into View Organizational & Contract Pricing

Synonym(s):
Amphotericin B – Low Endotoxin
UNSPSC Code:
12352207
NACRES:
NA.76

biological source

Streptomyces sp. (Microbial)

form

powder

mol wt

924.08

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

General description

Amphotericin B is a polyene antifungal antibiotic from Streptomyces sp. It has a high affinity for sterols,primarily ergosterols, of fungal and bacterial cell membranes.After binding to sterols, it forms channels in the membranes, causing small molecules to leak out.Amphotericin B is effective against fungi and yeast.The name of the drug is derived from the amphoteric behavior of the drug, due to the carboxyl group on the main ring and a primary amino group on the mycosamine ring.
Amphotericin B induces K+ leakage, which is separate from its lethal action, as was demonstrated in human erythrocytes and is due to the inhibitory effect on the Na+/K+ pump.At sub-lethal concentrations, this drug stimulates either the activity of some membrane enzymes or cellular metabolism,in particular stimulation of some cells of the immune system.

Preparation Note

If reconstituted at 25 mg/10 mL of sterile water, there is no need to filter sterilize. This will yield a slightly hazy yellow solution.

Storage and Stability

Amphotericin B remains active for 3 days in culture at 37°C. For long term, storage at 2−8°C, protected from air and light, is recommended. Under these conditions the products remain active for 5 years.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

STOT RE 1

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

Prevent fungal, yeast, and mold contamination in cell cultures. Discover the best antifungal agent for your cultures with the extensive Sigma® antifungal collection.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service