This technique is based on the direct proportionality of mass fraction ratio and signal intensity ratio of the natural isotope and the C-13 labeled caffeine.

Clinical Translation of Combined Hyperpolarized [^1-13C]pyruvate and [¹³C, ¹⁵N₂]urea MRI for Simultaneous Metabolic and Perfusion Imaging

This webinar discusses advances in the use of stable isotope labeled tracers during hyperpolarization and MRI to follow perfusion and metabolism in cancer cells.

Performing a Purification of IgG Antibodies with Protein G Sepharose® 4 Fast Flow

This page shows how to separate IgG antibodies by afﬁnity chromatography using Protein G Sepharose 4 Fast Flow from Cytiva.

Colorimetric Detection of Western blot using 4-Chloro-1-Naphthol Solution Protocol

This solution is used as a substrate for detecting horseradish-peroxidase conjugates in Western blotting.

Antibiotic Analysis Tetracyclines Sulfonamides Quinolones

LC-MS/MS-based determination of 19 sulfonamides, 13 quinolones, and 3 tetracycline drug residues in pork meat samples using Supel™ Swift HLB for extraction.

Neurotensin Receptors

Neurotensin is a 13 amino acid regulatory peptide found mainly in gut and brain. Intestinal neurotensin is produced by a discrete population of endocrine cells (N cells) scattered throughout the jejuno-ileal mucosa.

Immunoprecipitation of FLAG Fusion Proteins Using Monoclonal Antibody Affinity Gels

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

Nickel Carbonyl

Analysis of nickel carbonyl, nickel carbonyl in air, organometallics in air, exposure limits for nickel carbonyl, ni(co)4

Derivatization Agents for LC/MS – An Improved Detection of Estradiol with ESI-MS

Improved LC/MS Detection of Estradiol using 4-(Dimethylamino) benzoyl chloride (DMABC) derivatization agent relative to dansyl chloride.

Chromolith® WidePore 300 HPLC Columns

Analytical Chromolith® HPLC columns for Bio applications, 300A mesopores: RP-18, RP-8, RP-4, Prot-A, Epoxy

Software Simplifies Compliance with 21 CFR Part 11 and EudraLex Good Manufacturing Practice Volume 4 Annex 11

How software can simplify 21 CFR Part 11 and EudraLex Volume 4 Annex 11 compliance and provide additional benefits such as access control, audit trails and easier search and retrieval.

Heavy Metal Analysis of Cannabis sativa by ICP-MS and the Need for Proper Sample Homogenization

A 4-step comprehensive ICP-MS workflow including sample homogenization, with standard addition calibration method, to determine heavy metals in Cannabis sativa.

ELISA Protocols

This article offers 4 popular ELISA protocols: Sandwich ELISA protocol, Phosphorylation Assay Procedure, EIA Assay Procedure, & Cell-based Assay Procedure.

Complete Whole Transcriptome Amplification Kit Protocol (WTA2)

WTA2, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3-bias

Purification using Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, and Glutathione Sepharose® 4B

This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.

In Vitro Differentiation of Human iPS Cells into Airway Epithelial Lung Organoids for Respiratory Disease Research Applications

Lung organoids are valuable 3D models for human lung development and respiratory diseases. The 3dGRO™ differentiation protocol generates organoids from human iPSCs in 4 steps.

The Formulation Box - Biomolecules Stabilization and Aggregation Prevention

You are cordially invited to discover in 4 steps how you can improve your strategies for biomolecules formulation development and extend your knowledge about viscosity reduction capabilities

The Formulation Box - Biomolecules Stabilization and Aggregation Prevention

You are cordially invited to discover in 4 steps how you can improve your strategies for biomolecules formulation development and extend your knowledge about viscosity reduction capabilities

Enzymatic Assay of Cathepsin B

Cathepsin B is a lysosomal cysteine proteinase with broad specificity. This protocol uses Nα–CBZ–Arg–Arg–7–amido–4–methylcoumarin as the substrate for fluorometric detection of Cathepsin B activity.

Coupling Protocol for Primary Amine of a Ligand

This page shows coupling through the primary amine of a ligand with a NHS-activated Sepharose High Performance, NHS-activated Sepharose 4 Fast Flow and CNBr-activated Sepharose from Cytiva.

Removal of Detergents from Biological Product Matrices

The Amberlite™ XAD-4 resin used in Porozorb™ cartridges is a proven technology that is highly effective in removing various detergents from cell culture media for biopharmaceutical applications such as vaccine production.

Purifcation or Removal of Serine Proteases, e.g. Thrombin and Trypsin, and Zymogens

This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from Cytiva.

Titripac® Sustainable Packaging for Ready-to-Use Certipur® pH Buffers

Learn about our ready-to-Use Certipur® pH Buffer solutions (4 L & 10 L), available in an innovative Titripac® sustainable packaging for a wide pH range of 1 to 12.

Purification Using GST HiTrap® 1 mL and 5 mL Columns: GSTrap™ 4B, GSTrap™ FF, and GSTrap™ HP

GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.

Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode

This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification

Page 1 of 37