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p53 And Related Products
, IF, IP, PS
100 mg
Anti-p53 (Ab-4) (Wild type)
Mouse mAb (PAb246)
OP32 Liquid, purified . Immunogen used was BALB/c SVA31 E7 cells . Recognizes the ~53 kDa wild-type p53
protein in its native
The Effective Use of Protein Kinase Inhibitors
109 21 42 33 84 39 83 106 84 6 6 4 86 113 99 83 60 52 78 107 107
100 107 106 103 41 19 84 22 94 57 15 53 45 65 75 93 43 59
Application Note - RealTime PCR Quantification of Plant miRNAs Using Universal ProbeLibrary Technology
uo
re
sc
en
ce
(
53
0
nm
)
8.7
7.9
7.1
6.3
5.5
4.7
3.9
3.1
2.2
1.5
0.7
Cycle number
Amplification curves miR 166
Fl
uo
re
sc
en
ce
(
53
0
nm
)
9.9
9.0
8.1
The Effective Use of Protein Kinase Inhibitors
109 21 42 33 84 39 83 106 84 6 6 4 86 113 99 83 60 52 78 107 107
100 107 106 103 41 19 84 22 94 57 15 53 45 65 75 93 43 59
Plant Growth
Z 3541
D
(N6-(trans-4-Hydroxy-3-methyl-2-buten-1-
yl)adenosine; 9-(b-D-Ribofuranosyl)-trans-
zeatin)
CAS No. 6025-53-2
C15H21N5O5 FW 351.4
Plant cell culture, tested, approx.95%
Growth Regulator Detection
Data Sheet - SRP5049
, 0.1 mM PMSF and 25% glycerol.
Molecular mass: ∼74 kDa
Purity: 70–95% (SDS-PAGE, see Figure 1)
Specific Activity: 39–53 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product
Simple process strategies to increase the utilization of Protein A media in clinical manufacturing
ProSep® Ultra Plus
Single Flow
80 45 -
ProSep® Ultra Plus
Single Flow
95 54 20%
Eshmuno® A
Dual Flow
95 53 18%
0%
5%
10%
15%
pIgG mAb A mAb
MILLIPLEX®MAP Human Myokine Panel is an optimized quantitative immunoassay that simultaneously measures 15 novel muscle-secreted factors
35 21 163 89 65 143 44 71 31 88 46 161 71
std 3 212 150 94 58 354 227 157 310 133 155 53 243 95 258 333
std 4 750 849 307 411 1,154 736
Data Sheet - SRP5165
Figure 1.
SDS-PAGE Gel of Typical Lot
70–95% (densitometry)
References
1. Tang, S.C. BAG-1, an anti-apoptotic tumour
marker. IUBMB Life, 53(2), 99–105 (2003).
2. Clemo, N.K. et al., The role
Eppendorf Mastercyclers Save Time and Cost in PCR
Temperature Duration Temperature Duration
i-Taq
1 Initial denaturation 95°C 5 min 95°C 5 min
2
(30 cycles)
Denaturation 95°C 15 s 95°C 15 s
Annealing aGradient °C 30 s
bGradient°C 60 s
Extension
Data Sheet - SAB4200868
2019).
2. Ai Zu Tan C., et al., Curr Opin Microbiol., 53,
9-18 (2020).
3. Heikkila MP., et al., J Appl Microbiol., 95,
471–78 (2003).
4. Cebrian R., et al., Food Microbiol. 30.,
FcFusionProteinSeparation
5
10
15
20
8.
81
5
11
.4
80
12
.0
30
12
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25
13
.5
43
14
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14
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44
15
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16
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53
Data Sheet - SRP5169
apoptosis in a
spatially and temporally dependent manner in
human cells. Cell Stress Chaperones, 14, 343-53
(2009).
FF 11/11-1
2011 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark
Eppendorf Mastercyclers as robust PCR systems for GC-rich templates
Technologies SimpliAmp™ 4°C/s
Bio-Rad T100 4°C/s
Table 1: List of thermal cyclers in this study
30 cycles
Step Temperature Duration
i-Taq KAPA2G ATG360
Initial denaturation 95°C 5 min 5 min 10
Data Sheet - SRP5015
0.1 mM PMSF, and 25% glycerol.
Molecular mass: ∼72 kDa
Purity: 70–95% (SDS-PAGE, see Figure 1)
Specific Activity: 39–53 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product
Data Sheet - SRP5101
, 0.1 mM PMSF, and 25% glycerol.
Molecular mass: ∼82 kDa
Purity: 70–95% (SDS-PAGE, see Figure 1)
Specific Activity: 53–71 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product
Amicon® Ultra-4 Centrifugal Filter Devices Non IVD User guide
613 97 42 23 53
15 – 54 30 – 30
20 170 35 22 15 26
25 118 – – – –
30 92 – – – –
40 62 – – – –
Spin conditions: 7,500 × g for 3K 10K, 30K
Data Sheet - SAB4200862
Broz P., et al., Gut Microbes, 3: 62–70 (2012).
9. Haraga A., et al., Nat Rev Microbiol., 6:
53–66 (2008).
10. Lopez FE., et al., Food Res Int., 45:
842–51 (2012).
11.
Product Information Sheet - SBR00073
for Eluent A & B.
Time (min) A [%] B [%]
0 47 53
2 47 53
6 35 65
16 35 65
20 0 100
Data Sheet - p5221
Initial-denaturation 95°C 3 min
Denaturation 95°C 15 sec
35
Extension 72°C 30 sec/kb
Final Extension 72°C 5 min
Reliable High Fidelity
The amplification fidelity of Acculong DNA
Polymerase is 53-fold superior
Data Sheet - d5121
Initial-denaturation 95°C 3 min
Denaturation 95°C 15 sec
35
Extension 72°C 30 sec/kb
Final Extension 72°C 5 min
Reliable High Fidelity
The amplification fidelity of Acculong DNA
Polymerase is 53-fold superior
Spare parts for Mobius®Bioreactors (50 L, 200 L, 1000 L & 2000 L)
86
1
5
76
50
97
96 87
94
95
76
2
84
78
79
51
3
80
82
4
Amicon® Ultra-4 Centrifugal Filter Devices User Guide
613 97 42 23 53
15 – 54 30 – 30
20 170 35 22 15 26
25 118 – – – –
30 92 – – – –
40 62 – – – –
Spin conditions: 7,500 × g for 3K 10K, 30K
Data Sheet - E9769
protein-2� and macrophage inflammatory
protein-2�. ENA-78 shares 53% sequence homology1
with NAP-2 and 52% sequence homology with GRO�.1
ENA-78 shares several properties of neutrophil
activation
Aldrich Polymer Products Application - Transitions
methacrylate 20
tert-Butyl methacrylate 118
4-tert-Butylstyrene 127
tert-Butyl vinyl ether 88 250
Butyl vinyl ether -55 64
ε-Caprolactone -60
Cellulose nitrate 53
Cellulose tripropionate
cis-Chlorobutadiene
Data Sheet - F6027
sent to the
53-7.3 reacts with cells of all mouse strains tested. attention of the safety officer of your institution.
Performance
When assayed by flow cytometry, approximately 1 µg
(4 µl) of the
Product Information Sheet - P8092
and thermocycler.
Common cycling parameters are:
a. Denature the template at 95 °C for 1-2 minutes
b. Anneal primers at 53 °C for 1 minute
c. Extension at 72 °C for 1 minute
25-30 cycles of amplification
Data Sheet - P9217
and thermocycler.
Common cycling parameters are:
a. Denature the template at 95 °C for 1-2 minutes
b. Anneal primers at 53 °C for 1 minute
c. Extension at 72 °C for 1 minute
25-30 cycles of amplification
Monoclonal Anti-CDw75 antibody produced in mouse (C3080)
1984).
2. Bast, B., et al., J. Cell Biol., 116, 423
(1992).
3. Okon, E., et al., Cancer, 56, 95 (1985).
4. Marder, R., et al., Lab. Invest., 52, 497
(1985).
5. Linder, J., In: Advances in
Immunohistochemistry
Rapid Identification of coliform bacteria and E. Coli in water
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