UHPLC/MS Analysis of 8-Hydroxy-2′-Deoxyguanosine and Related Compounds on Titan Silica

column

Titan Silica, 10 cm x 3.0 mm I.D., 1.9 μm particles (581536-U)

mobile phase

[A] acetonitrile; [B] 100 mM ammonium formate, pH 3.0 with formic acid; (90:10, A:B)

flow rate

0.6 mL/min

pressure

2060 psi (142 bar)

column temp.

35 °C

detector

MS ESI+, combined SIR, 268, 284 m/z

injection

2 μL

sample

1 μg/mL in 99:1, acetonitrile:water