HomePhotometry & ReflectometryAmmonium in Effluents

Ammonium in Effluents

Photometric determination using the indophenol blue method

Two scientists testing the water at a river.


Ammonium is a key parameter in water testing, as it can have adverse effects on marine ecosystems if it is at high levels. One major issue is eutrophication, excessive plant and algal growth.1 As a result, determining ammonium levels in effluents is critical to maintaining aquatic environments. In this application note, we describe the quantification of ammonium in effluents using the Spectroquant® photometric system with ammonium test kits.

Experimental Method

Ammonium nitrogen (NH4-N) occurs partly in the form of ammonium ions and partly as ammonia. A pH-dependent equilibrium exists between the two forms. In strongly alkaline solution ammonium nitrogen is present almost entirely as ammonia, which reacts with hypochlorite ions to form monochloramine. This in turn reacts with a substituted phenol to form a blue indophenol derivative that is determined photometrically. Due to the intrinsic yellow coloration of the reagent blank, the measurement solution is yellow green to green in color.

The method is analogous to EPA 350.1, APHA 4500-NH3 F, ISO 7150-1, and DIN 38406-5.

Reagents, Instruments and Materials

Test Kits

One of the following test kits is necessary for this analysis

  • Ammonium Cell Test method: photometric 0.010 - 2.000 mg/l NH4-N; 0.01 - 2.58 mg/l NH4+ Spectroquant® (1.14739)
  • Ammonium Cell Test Method: photometric 0.20 - 8.00 mg/l NH4-N; 0.26 - 10.30 mg/l NH4+ Spectroquant® (1.14558)
  • Ammonium Cell Test Method: photometric 0.5 - 16.0 mg/l NH4-N; 0.6 - 20.6 mg/l NH4+ Spectroquant® (1.14544)*
  • Ammonium Cell Test Method: photometric 4.0 - 80.0 mg/l NH4-N; 5.2 - 103.0 mg/l NH4+ Spectroquant® (1.14559)
  • Ammonium Test Method: photometric 0.010 - 3.00 mg/l NH4-N; 0.013 – 3.86 mg/l NH4+ Spectroquant® (1.14752)
  • Ammonium Test Method: photometric 2.0 - 150 mg/l NH4-N; 2.6 - 193 mg/l NH4+ Spectroquant® (1.00683)

*Not compatible with Move 100


One of the following instruments is necessary for this analysis

  • Spectroquant® VIS Spectrophotometer Prove 100 (173016)
  • Spectroquant® UV/VIS Spectrophotometer Prove 300 (173017)
  • Spectroquant® UV/VIS Spectrophotometer Prove 600 (173018)
  • Spectroquant® Photometer NOVA 60A (1.09752)**
  • Spectroquant® Colorimeter Move 100 (1.73632)

** This analysis pertains to Nova 60A and all legacy Nova instruments (Nova 30 or Nova 60)


  • Rectangular cells 10 mm (1.14946) and/or
  • Rectangular cells 20 mm (1.14947) and/or
  • Rectangular cells 50 mm (1.14944)

Sample preparation

Cloudy samples must be filtered before determination.


Determine with the above-mentioned test kits.


Ammonium content in mg/l NH4-N = analysis value in mg/l NH4-N



Camargo JA, Alonso Á. 2006. Ecological and toxicological effects of inorganic nitrogen pollution in aquatic ecosystems: A global assessment. Environment International. 32(6):831-849.