Photometric determination using the Phosphorus molybdenum blue method.
Phosphorous is a key nutrient that must be kept within certain limits in the environment. In excess, it can upset aquatic ecosystems by encouraging excessive growth of algae and plants in a process termed eutrophication.1 As a result, testing phosphorous in effluents is critical from an environmental perspective. In this application note, we describe a determination of one form of phosphorous, phosphate, using photometry and Spectroquant® Phosphate Test Kits.
In sulfuric solution, orthophosphate ions react with molybdate ions to form molybdophosphoric acid. Ascorbic acid reduces this to phosphomolybdenum blue (PMB) that is determined photometrically. The method is analogous to EPA 365.2+3, APHA 4500-P E, and DIN EN ISO 6878.
One of the following test kits is necessary for this analysis:
One of the following instruments is necessary for this analysis:
* This analysis pertains to Nova 60A and all legacy Nova instruments (Nova 30 or Nova 60)