Merck
All Photos(1)

480708

Sigma-Aldrich

α2-3,6-Neuraminidase, Clostridium perfringens, Recombinant, E. coli

Synonym(s):
α2-3,6-Neuraminidase, Clostridium perfringens, Recombinant, E. coli, Acetylneuraminyl Hydrolase, Sialidase
CAS Number:
Enzyme Commission number:

recombinant

expressed in E. coli

Quality Level

product line

EMPROVE® EXPERT

form

liquid

specific activity

≥10 units/mL
≥250 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

foreign activity

N-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α- and β-mannosidase, proteases, none detected

shipped in

wet ice

storage temp.

2-8°C

General description

Note: 1 mU = 1 milliunit.
Recombinant, Clostridium perfringens α2-3,6-Neuraminidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal α2,3 and α2,6 unbranched sialic acid residues from complex carbohydrates and glycoproteins. This enzyme does not exhibit activity on α2,8 or branched sialic acids.

Packaging

500 miu in Plastic ampoule

Application

Recommended reaction buffer: 50 mM sodium phosphate buffer, pH 6.0

Warning

Toxicity: Harmful (C)

Unit Definition

One unit is defined as the amount of enzyme that will catalyze the release of 1 µmol of methylumbelliferone from 2ʹ-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic Acid (Cat. No. 474495) per min at 37°C, pH 5.0.

Physical form

In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.

Other Notes

Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
Ohta, Y., et al. 1989. J. Biochem (Tokyo) 106, 1086.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

CorrosionExclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

8B - Non-combustible, corrosive hazardous materials

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Quality

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