14510

1,2-Bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid

≥96.0% (HPLC)

• Synonym(s): BAPTA, Ethylenedioxybis(o-phenylenenitrilo)tetraacetic acid
• Empirical Formula (Hill Notation): C₂₂H₂₄N₂O₁₀
• CAS Number: 85233-19-8
• Molecular Weight: 476.43
• Beilstein: 5192406
• MDL number: MFCD00036255
• PubChem Substance ID: 329750514
• NACRES: NA.22

Properties

• Quality Level: 200
• Assay: ≥96.0% (HPLC)
• form: powder
• fluorescence: λ_ex 251 nm; λ_em 363 nm (pH 8.0 Highly selective calcium chelating reagent. Suitable for the spectrophotometric monitoring of Ca2+ levels); λ_ex 274 nm; λ_em 363 nm (With Calcium)
• storage temp.: 2-8°C
• SMILES string: OC(=O)CN(CC(O)=O)c1ccccc1OCCOc2ccccc2N(CC(O)=O)CC(O)=O
• InChI: 1S/C22H24N2O10/c25-19(26)11-23(12-20(27)28)15-5-1-3-7-17(15)33-9-10-34-18-8-4-2-6-16(18)24(13-21(29)30)14-22(31)32/h1-8H,9-14H2,(H,25,26)(H,27,28)(H,29,30)(H,31,32)
• InChI key: FTEDXVNDVHYDQW-UHFFFAOYSA-N

Description

General description

1,2-Bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid is a highly selective calcium chelating reagent. Effect of microinjected calcium-loaded 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid (CaBAPTA) on cell-to-cell diffusion of carboxyfluorescein has been studied in staminal hairs of Setcreasea purpurea. It is reported to be a metabolite of 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid tetra(acetoxymethyl ester) (BAPTA-AM) in rat urine.

Application

1,2-Bis(2-aminophenoxy)ethane-N,N,N′,N′′-tetraacetic acid was used for the spectrophotometric monitoring of Ca²⁺ levels. It may be used as chelator of cytosolic Ca²⁺ to investigate the effect of methoxychlor on cytosolic free Ca²⁺ concentrations and apoptosis in HA59T human hepatoma cells.

Highly selective calcium chelating reagent. Suitable for the spectrophotometric monitoring of Ca²⁺ levels

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves