A7721

Sigma-Aldrich

JumpStart Taq Antibody

Adds hot-start capabilities to any Taq DNA Polymerase

MDL number:

Quality Level

form

liquid

feature

hotstart

concentration

1.1 mg/mL

color

colorless

conjugate

unconjugated

shipped in

wet ice

storage temp.

−20°C

Related Categories

Application

JumpStartTaq Antibody is suitable for use in:
  • cytochrome b locked nucleic acid (LNA) probe assay
  • ligation-mediated polymerase chain reaction (LM-PCR)
  • in Real-Time quantitative PCR Analysis of viral titer

The primary purpose of all hot start PCR methods is to prevent Taq DNA polymerase activity prior to thermal cycling. Even if set-up is conducted on ice, the Taq DNA polymerase remains active and may elongate unwanted products such as misprimed or other non-specific events. Primer-dimer interactions may also be amplified, which will reduce overall yield and efficiency of desired products.

One way to prevent unwanted amplification products is to add JumpStart Taq Antibody to the reaction. This efficient yet simple procedure takes only 10 minutes and effectively inactivates the Taq DNA polymerase until the first denaturation cycle. Upon heating to 70 °C, the antibody dissociates and full activity is restored to the Taq DNA polymerase for the remainder of the PCR. Unlike chemically inactivated hot-start methods, no extended heating step is required for reactivation with the use of JumpStart Taq Antibody.

JumpStart Taq antibody works effectively on a variety of commercially available Taq DNA polymerases.

Features and Benefits

  • Minimize non-specific amplification while increasing target yield & specificity
  • Reduce set-up time and eliminate concerns associated with manual or wax hot-start methods.

Packaging

Supplied with dilution buffer.

Components

JumpStart Taq Antibody, 1.1μg/1μL
Dilution Buffer provided in 1 mL vials

Quantity

Two units of Taq DNA polymerase are inactivated by 1 test of JumpStart Taq Antibody.

Other Notes

View more detailed information on JumpStart Taq enzymes at www.sigma-aldrich.com/hotstart.

Legal Information

JumpStart is a trademark of Sigma-Aldrich Co. LLC

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 3

Flash Point F

Not applicable

Flash Point C

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Enhanced gene targeting of adult and pluripotent stem cells using evolved adeno-associated virus
Gene correction, 169-179 (2014)
Measuring and monitoring apoptosis and drug toxicity in HIV patients by ligation-mediated polymerase chain reaction
Hooker DJ, et al.
Journal of Cellular and Molecular Medicine, 13(5), 948-958 (2009)
Application of real-time and multiplex polymerase chain reaction assays to study leaf blotch epidemics in barley
Fountaine JM, et al.
Phytopathology, 97(3), 297-303 (2007)
D J Sharkey et al.
Bio/technology (Nature Publishing Company), 12(5), 506-509 (1994-05-01)
We demonstrate the utility of antibodies to the DNA polymerase from Thermus aquaticus (TaqPol) as thermolabile inhibitors of TaqPol activity. One of the limitations of the polymerase chain reaction (PCR) is the co-amplification of non-specific products caused by TaqPol activity...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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