As mentioned in the 'PROPERTIES' section, the volume of each vial is 1.5 mL.
W1754
Water
PCR Reagent, suitable for PCR
Synonym(s):
H2O
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 vial | Please contact Customer Service for Availability | NOK 446.00 | |
| 5 vials | Please contact Customer Service for Availability | NOK 1,540.00 |
About This Item
Linear Formula:
H2O
CAS Number:
Molecular Weight:
18.02
NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12191602
EC Number:
231-791-2
MDL number:
Beilstein/REAXYS Number:
2050024
Technique(s):
PCR: suitable
Bp:
100 °C (lit.)
Vapor pressure:
3 mmHg
NOK 446.00
Please contact Customer Service for Availability
Product Name
Water, PCR Reagent
vapor density
<1 (vs air)
Quality Level
vapor pressure
3 mmHg
sterility
sterile-filtered
form
liquid
packaging
vial of 1.5 mL
technique(s)
PCR: suitable
refractive index
n20/D 1.34 (lit.)
pH
5-7
bp
100 °C (lit.)
mp
0 °C (lit.)
density
1.000 g/mL at 3.98 °C (lit.)
foreign activity
DNase, none detected, RNase, none detected
SMILES string
O
InChI
1S/H2O/h1H2
InChI key
XLYOFNOQVPJJNP-UHFFFAOYSA-N
General description
PCR grade water is sterile-filtered. It is free from exonucleases (DNAse, RNAse) and endonuclease (NICKase) and is also free from nucleic acid contamination.
Application
Suitable for polymerase chain reaction (PCR)
Water has been used:
- as a component of the reaction mixture and as a diluting agent in microfluidic RT-qPCR
- as a component of the reaction mixture for the amplification of products from fungal (Trametes versicolor) DNA
- as a diluting agent and as a component of the reaction mixture for the amplification of cDNA[1]
- Water has been used to make up the final volume of the sample in polymerase chain reaction (PCR)[1]
Other Notes
Easily compare specifications for Water products with the Water specification table.
1 of 1
This Item | |||
|---|---|---|---|
| technique(s) PCR: suitable | technique(s) FISH: suitable | technique(s) - | technique(s) - |
| Quality Level 200 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| form liquid | form liquid | form liquid | form liquid |
| pH 5-7 | pH 5-7 | pH - | pH - |
| bp 100 °C (lit.) | bp 100 °C (lit.) | bp 100 °C (lit.) | bp 100 °C (lit.) |
| vapor density <1 (vs air) | vapor density - | vapor density - | vapor density - |
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Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Protocols
Optimization of qPCR conditions is important for the development of a robust assay. The two main approaches are optimization of primer concentration and/or annealing temperatures.
SYBR Green I dye in qPCR measures target quantity, adaptable to specific needs with a standard protocol.
Analysis of gene expression data requires a stable reference or loading control. This reference is usually one or more reference genes.
Articles
Small interfering RNAs (siRNAs) are powerful tools for gene expression knockdown, widely used in molecular biology.
Francine Marciano-Cabral et al.
Applied and environmental microbiology, 69(10), 5864-5869 (2003-10-09)
The free-living amoeboflagellate Naegleria fowleri is the causative agent of primary amoebic meningoencephalitis (PAM), a rapidly fatal disease of the central nervous system. In the United States, the disease is generally acquired while swimming and diving in freshwater lakes and
J Loeffler et al.
Journal of clinical microbiology, 37(4), 1200-1202 (1999-03-13)
Successful in vitro amplification of fungal DNA in clinical specimens has been reported recently. In a collaboration among five European centers, the frequency and risk of contamination due to airborne spore inoculation or carryover contamination in fungal PCR were analyzed.
Circulating Tfh1 (cTfh1) cell numbers and PD1 expression are elevated in low-grade B-cell non-Hodgkin?s lymphoma and cTfh gene expression is perturbed in marginal zone lymphoma
Elliot T B, et al.
PLoS ONE (2018)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| W1754-5VL | 04061837488696 |
| W1754-1VL | 04061837488689 |
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容量がわかりません。型番の1 VLとは1000 mLのことですか？
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