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Z380059

Sigma-Aldrich

Breathe-Easy® sealing membrane

polyurethane membrane with acrylic adhesive pre-cut to fit standard multiwell plates.

Synonym(s):
breathable sealing membrane, microplates, plate sealers, microwell plates, sealing film, multi-well sealing film, multi-well plates, cell culture, plate sealing film, tissue culture
NACRES:
NB.22

material

rectangular transparent membrane (polyurethane)

sterility

sterile

feature

piercable

packaging

pack of 100 ea

General description

Breathe-Easy® membrane is permeable to oxygen, carbon dioxide and water vapor, creating separate, highly uniform microculture vessels from a multi-well plate.
The membrane comes as a sandwich. The bottom paper liner protects the adhesive layer, and the top carrier sheet protects the outer surface of the highly compliant membrane until it has been placed and adhered to the plate. The liners also assure sterility of each individual membrane. The adhesive seals every well (unlike molded plastic covers), permitting agitation without cross-contamination or microbial infiltration. Compatible with most caustics and organic solvents, stable from −80 to +100 °C.
Note: Use of the Brayer (Z380067 sold separately) is recommended to assure uniform adhesion and to facilitate removal of the top paper liner.

Features and Benefits

Breathe-Easy® memebrane:
  • Sterile & Non-Cytotoxic: Sterilized by gamma irradiation (free of cytotoxins)
  • Prevent Contamination: Films eliminate well-to-well contamination, microbial and viral contamination, evaporation (excluding breathable membranes) and sample spillage
  • Ideally Sized and Designed: 6" x 3.25" rectangles fit all microplates. Two scored end-tabs make film positioning and removal hassle-free
  • Thermal Stability: Designed to withstand a wide temperature range
  • Easy Specimen Retrieval: Pierceable sealing films facilitate easy removal of samples with a pipet tip

Legal Information

Breathe-Easy is a registered trademark of Diversified Biotech

Certificate of Analysis

Certificate of Origin

Victoria Hsiao et al.
ACS synthetic biology, 4(2), 150-161 (2014-05-23)
As a field, synthetic biology strives to engineer increasingly complex artificial systems in living cells. Active feedback in closed loop systems offers a dynamic and adaptive way to ensure constant relative activity independent of intrinsic and extrinsic noise. In this...
Teng Wang et al.
Nature communications, 11(1), 5589-5589 (2020-11-06)
Conjugative plasmids can mediate the spread and maintenance of diverse traits and functions in microbial communities. This role depends on the plasmid's ability to persist in a population. However, for a community consisting of multiple populations transferring multiple plasmids, the...
Adithi R Varadarajan et al.
NPJ biofilms and microbiomes, 6(1), 46-46 (2020-11-01)
Pseudomonas aeruginosa MPAO1 is the parental strain of the widely utilized transposon mutant collection for this important clinical pathogen. Here, we validate a model system to identify genes involved in biofilm growth and biofilm-associated antibiotic resistance. Our model employs a...
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Filipe Pinto et al.
Nature communications, 11(1), 1529-1529 (2020-04-07)
Inteins are protein segments capable of joining adjacent residues via a peptide bond. In this process known as protein splicing, the intein itself is not present in the final sequence, thus achieving scarless peptide ligation. Here, we assess the splicing...

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