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The Researcher’s Guide to Designing CRISPR and RNAi Screening Experiments
This screening guide covers how to choose a cell line, a screening library, and experimental conditions as well as tips for designing and performing your experiment.
Delivery of Nucleic Acids Using Polymers
This review will focus on the sub-sector of drug delivery concerning the delivery of nucleic acids, with a particular focus on poly(ethyleneimine).
siRNA Delivery in Mammalian Cell Lines
siRNAs are effectively delivered into mammalian cell lines that have been difficult or even impossible to transfect with siRNA when using other commercially available lipofection reagents.
esiRNA FAQs (Frequently Asked Questions)
esiRNA are endoribonuclease prepared siRNAs that target the same mRNA sequence for gene silencing. Here are some of the most asked questions regarding esiRNA uses and availability.
Single-Cell Resolution Discovery
CRISPR lentiviral screening libraries, partnered with 10x Genomics, offer powerful research tools for pooled screening.
Examination of Lentiviral Library Efficiency
Small interfering RNAs (siRNAs) are powerful tools for gene expression knockdown, widely used in molecular biology.
Predesigned siRNA
Rosetta siRNA Design Algorithm optimizes siRNA sequences for reduced off-target effects and increased RNAi performance.
Safe Lentivirus Handling
Lentiviral vector systems prioritize safety features, with design precautions preventing replication. Good handling practices are essential for use.
Experimental Design and Analysis — Experimental Design FAQs
We answer your frequently asked questions about designing shRNA experiments, including appropriate controls, screening techniques, selection and more.
miRNA (microRNA) Introduction
Mature microRNAs regulate gene expression through various mechanisms; partially complementary to mRNA molecules, downregulating gene expression.
Methods for Enhancing Lentiviral Transduction Efficiency
Methods for lentiviral transduction of Jurkat cells were compared. Spinoculation was compared with overnight incubation with polybrene (hexadimethrine bromide) and fibronection-coated plates.
Next-Gen RNA Interference with MISSION® shRNA
RNAi technology advancements enable gene expression silencing through endogenous RNAi pathways, applicable across eukaryotes.
Nuclease-Free Water at Your Fingertips
An ultrafiltration cartridge can be placed at the outlet of a water purification system to deliver nuclease-free ultrapure water.