MABC1152

Anti-LINE-1 ORF1p Antibody, clone 4H1

clone 4H1, from mouse

• Synonym(s): LINE-1 retrotransposable element ORF1 protein, L1ORF1p, LINE retrotransposable element 1, LINE1 retrotransposable element 1
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 4H1, monoclonal
• Application: ICC, IHC, IP, WB
• Citations: 44

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 4H1, monoclonal
• species reactivity: human
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG1κ
• UniProt accession no.: Q9UN81
• target post-translational modification: unmodified
• Gene Information: human ... L1RE1 (4029)

Description

General description

LINE-1 retrotransposable element ORF1 protein (UniProt: Q9UN81; also known L1ORF1p, LINE retrotransposable element 1, LINE1 retrotransposable element 1) is encoded by the LIRE1 (also known as LRE1) gene in human. LINE1-ORF 1P belongs to the transposase 22 family. It is mainly cytoplasmic protein and is enriched in stress granules, but may rarely be detected in the nucleolus. It contains a coiled coil domain that mediates its homotrimerization. The RNA recognition motif (RRM) the C-terminal domain are required for its proper RNA-binding activity. LINE1-ORF 1P is a nucleic acid-binding protein which is essential for retrotransposition of LINE-1 elements in the genome. It is also reported to function as a nucleic acid chaperone binding its own transcript and therefore preferentially mobilizing the transcript from which they are encoded. Retrotransposition is a shown to be a complex process that involves transcription of the LINE1, transport of its RNA to the cytoplasm, translation of the bicistronic RNA, formation of a ribonucleoprotein particle, its re-import to the nucleus and then target-primed reverse transcription at the integration site. Somatic LINE1 insertions are rare in most tissues, but are found in brain, testis, and many cancer types and have been implicated in carcinogenesis. Its expression is consistently detected in p53 mutant tumors, but, little or no expression is detected in Wilms tumors that were normal for p53. (Ref.: Goodier, JL et al. (2013). Nucleic Acids Res. 41(15): 7401-7419).

~42 kDa observed; 40.06 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Immunogen

A linear peptide corresponding to 10 amino aicds from the N-terminal region of human LINE-1 ORF1 protein.

Application

Detect LINE-1 retrotransposable element ORF1 protein using this mouse monoclonal Anti-LINE-1 ORF1p Anibody, clone 4H1, Cat. No. MABC1152. It is used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Immunohistochemistry Analysis: A representative lot detected LINE-1 ORF1p in Immunohistochemistry applications (Rodic, N., et. al. (2014). AM J Pathol. 184(5):1280-6; Doucet-O′Hare, T.T., et. al. (2015). Proc Natl Acad Sci USA. 112(35):E4894-900; Wylie, A., et. al. (2016). Genes Dev. 30(1):64-77).

Immunocytochemistry Analysis: A representative lot detected LINE-1 ORF1p in Immunocytochemistry applications (Taylor, M.S., et. al. (2013). Cell 155(5):1034-48).

Immunoprecipitation Analysis: A representative lot detected LINE-1 ORF1p in Immunoprecipitation applications (Taylor, M.S., et. al. (2013). Cell 155(5):1034-48).

Western Blotting Analysis: A representative lot detected LINE-1 ORF1p in Western Blotting applications (Taylor, M.S., et. al. (2013). Cell 155(5):1034-48).

Research Category
Apoptosis & Cancer

Biochem/physiol Actions

Clone 4H1 specifically detects LINE-1 ORF1 protein in MCF7 cells. This clone targets the sequence corresponding to 10 amino acids from the N-terminal region of LINE-1 ORF1p.

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in MCF7 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LINE-1 ORF1p in 10 µg of MCF7 cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable