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MRCF0R030

Microcon® Centrifugal Filters

NMWCO 30 kDa, Ultracel® regenerated cellulose membrane (low binding), sample volume 0.5 mL

Synonym(s):

Centrifuge concentrator

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Pack SizeSKUAvailabilityPrice
100 ea
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$1,000.00

About This Item

UNSPSC Code:
41104916
NACRES:
NB.24
eCl@ss:
36100101
Material:
Ultracel® regenerated cellulose membrane (low binding), polyacetal base (membrane support), polycarbonate device top, polypropylene tube (for filtrate/concentrate), silicone O-ring (medical-grade)
Pore size:
30 kDa NMWCO
Working volume:
0.5 mL
Sterility:
non-sterile
Packaging:
pkg of 100 ea

$1,000.00


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Quality Level

material

Ultracel® regenerated cellulose membrane (low binding), polyacetal base (membrane support), polycarbonate device top, polypropylene tube (for filtrate/concentrate), silicone O-ring (medical-grade)

sterility

non-sterile

feature

holdup volume> 10 μL

packaging

pkg of 100 ea

manufacturer/tradename

Microcon®

parameter

0.5 mL sample volume

technique(s)

DNA purification: suitable, RNA purification: suitable, protein extraction: suitable, protein purification: suitable

L

4.50 cm (1.8 in.)

diam.

12.3 mm

filtration area

0.32 cm2

working volume

0.5 mL

pore size

30 kDa NMWCO

shipped in

ambient

General description

Simply and efficiently concentrate and desalt solutions of any macromolecule, using any centrifuge that can accept 1.5 mL tubes. With the low-binding Ultracel membrane, Microcon filters offer:
•Typical recoveries of greater than 95%, even for dilute solutions
•Reverse spin to maximize recovery, even in the smallest samples
•Convenient storage of filtrate or concentrated sample in standard microfuge tube
•Concentration factors up to 100X

Features:
•High recovery for small volumes with reverse spin (concentration factor less than 20X)
•Low-binding Ultracel membrane
•Fast processing

Sample applications:
•Recovery of genomic DNA for forensic applications
•Concentration and desalting of nucleic acids (high recovery alternative to ethanol precipitation)
•Removal of primers from amplified DNA
•Easy filtrate recovery, exploiting the flat membrane design

Other Notes

Replaces: 42410

Legal Information

MICROCON is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

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This Item
MRCPRT010MRCF0R100MRCF0R100ET
parameter

0.5 mL sample volume

parameter

0.5 mL sample volume

parameter

0.5 mL sample volume

parameter

0.5 mL sample volume

pore size

30 kDa NMWCO

pore size

10 kDa NMWCO

pore size

-

pore size

-

manufacturer/tradename

Microcon®

manufacturer/tradename

Microcon®

manufacturer/tradename

Microcon®

manufacturer/tradename

Microcon®

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

material

Ultracel® regenerated cellulose membrane (low binding), polycarbonate device top, silicone O-ring (medical-grade), polyacetal base (membrane support), polypropylene tube (for filtrate/concentrate)

material

Ultracel® regenerated cellulose membrane (low binding), polyacetal base (membrane support), polycarbonate device top, polypropylene tube (for filtrate/concentrate), silicone O-ring (medical-grade)

material

Ultracel® regenerated cellulose membrane (low binding), polyacetal base (membrane support), polycarbonate device top, polypropylene tube (for filtrate/concentrate), silicone O-ring (medical-grade)

material

Ultracel® regenerated cellulose membrane (low binding), polyacetal base (membrane support), polycarbonate device top, polypropylene tube (for filtrate/concentrate), silicone O-ring (medical-grade, dual cycle ethylene oxide (EtO) treated)

working volume

0.5 mL

working volume

0.5 mL

working volume

0.5 mL

working volume

0.5 mL


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Storage Class

10-13 - German Storage Class 10 to 13



Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

An optimized LC-MS/MS based workflow for low artifact tryptic digestion and peptide mapping of monoclonal antibody, adalimumab (Humira) using filter assisted sample preparation (FASP).

A step-by-step protocol for released N-linked glycan analysis of the monoclonal antibody adalimumab, based on UHPLC-FLR-MS and procainamide labeling.

Articles

Method development for protein fingerprinting of AAV serotype 5 using both intact mass analysis and peptide mapping, to determine critical quality attributes for gene therapy, utilizing three different columns.

This application note describes the released N-Glycan analysis of a monoclonal antibody, cetuximab, labeled with procainamide, using a BIOshell™ Glycan HPLC column.

An optimized peptide mapping protocol using NISTmAb as a model monoclonal antibody, shorter incubation times, and improved digestion buffer to demonstrate minimal artificial asparagine deamidation and methionine oxidation.

View All Articles

Related Content

Microcon® Centrifugal Filters simply and efficiently concentrate and desalt solutions of DNA, RNA, protein or other macromolecules, using any centrifuge that can accept 1.5 mL tubes.


Flavio Oliveira et al.
Toxins, 12(3) (2020-04-05)
Toxic cyanobacterial blooms are a major contaminant in inland aquatic ecosystems. Furthermore, toxic blooms are carried downstream by rivers and waterways to estuarine and coastal ecosystems. Concerning marine and estuarine animal species, very little is known about how these species
Michael D Ward et al.
Clinical proteomics, 17, 11-11 (2020-03-21)
Detection of viral ribo-nucleic acid (RNA) via real-time polymerase chain reaction (RT-PCR) is the gold standard for the detection of Ebola virus (EBOV) during acute infection. However, the earliest window for viral RNA detection in blood samples is 48-72 h post-onset
Sarah K Stumper et al.
eLife, 8 (2019-02-06)
RNA polymerases (RNAPs) contain a conserved 'secondary channel' which binds regulatory factors that modulate transcription initiation. In Escherichia coli, the secondary channel factors (SCFs) GreB and DksA both repress ribosomal RNA (rRNA) transcription, but SCF loading and repression mechanisms are



Global Trade Item Number

SKUGTIN
MRCF0R03004053252579707

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