ZRB2683

Anti-Renilla Luciferase Antibody, clone 3G19 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Sinónimos: Coelenterazine h 2-monooxygenase, EC:1.13.12.5, Renilla-luciferin 2-monooxygenase, Renilla-type luciferase
• Código UNSPSC: 12352203

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 3G19, recombinant monoclonal
• descripción: recombinant, expressed in HEK 293 cells
• Línea del producto: ZooMAb^® learn more
• Formulario: lyophilized
• mol peso: calculated mol wt 36.02 kDa; observed mol wt ~50 kDa
• purificado por: using Protein A
• reactividad de especies (predicha por homología): invertebrates
• envase: antibody small pack of 25
• características de los productos alternativos más sostenibles: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validación mejorada: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• técnicas: ELISA: suitable; affinity binding assay: suitable; immunocytochemistry: suitable; western blot: suitable
• isotipo: IgG
• secuencia del epítopo: Unknown
• Nº de acceso UniProt: P27652
• categoría alternativa más sostenible: Aligned
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Especificidad

Clone 3G19 is a ZooMAb^® recombinant rabbit monoclonal antibody that specifically detects Renilla-type luciferase.

Inmunógeno

His-tagged, full length, recombinant Renilla Luciferase.

Aplicación

Quality Control Testing

Evaluated by Western Blotting with His-tagged, full length, recombinant Renilla Luciferase.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected His-tagged, full length, recombinant Renilla Luciferase.

Tested Applications

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Renilla Luciferase in HeLa cells transfected with Renilla luciferase.

Enzyme Immunoassay: A representative lot of this antibody detected Renilla Luciferase protein in ELISA application.

Affinity Binding Assay: A representative lot of this antibody bound Renilla Luciferase with at least ten thousand-fold (10,000X) higher than with non-specific GST control protein.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descripción de destino

Coelenterazine h 2-monooxygenase (UniProt: P27652; also known as EC:1.13.12.5, Renilla-luciferin 2-monooxygenase, Renilla-type luciferase) is a monomeric, bioluminescent protein derived from the sea pansy (Renilla reniformis). Due to its light emitting abilities, the luciferase from Renilla reniformis has been widely used in molecular biology as a reporter gene in cell culture experiments and small animal imaging. Renilla luciferase is shown to be present in membrane-bound intracellular structures within specialized light-emitting cells along with a closely interacting green fluorescent protein (RrGFP) and a calcium- activated luciferin-binding protein. Upon binding the substrate, it catalyzes an oxygenation reaction, producing a very short-lived hydroperoxide that cyclizes into a dioxetanone structure that collapses, releasing a CO2 molecule. The spontaneous breakdown of the dioxetanone releases the energy that is necessary to generate the excited state of the coelenteramide product, which is the singlet form of the monoanion. The optimum pH for this reaction is 7.4. It is shown that under in vivo conditions the product undergoes the process of nonradiative energy transfer to an accessory protein, a GFP, which results in green bioluminescence (~ 509 nm). However, in vitro, in the absence of GFP, it emits blue light (~480 nm). (Ref.: Loening, AM., et al. (2007). J. Mol. Biol. 374(4); 1017-1028; Lorenz, WW., et al. (1991). Proc. Natl. Acad. Sci. USA 88(10); 4438-4442).

Forma física

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitución

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Información legal

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable