DNA duplex stabilization in crowded polyanion solutions.

DNA duplex stabilization in crowded polyanion solutions.

Chemical communications (Cambridge, England) (2013-01-11)

Imran Khimji, Jeehae Shin, Juewen Liu

ABSTRACT

The melting temperature of duplex DNA is much higher in polyanions than in non-ionic polymers with similar ionic strength, suggesting an additional electrostatic contribution on top of the excluded volume effect.

MATERIALS

Product Number

Brand

Product Description

523925

Sigma-Aldrich

Poly(acrylic acid) solution, average M_w ~100,000, 35 wt. % in H₂O

416002

Sigma-Aldrich

Poly(acrylic acid) solution, average M_w ~250,000, 35 wt. % in H₂O

535931

Sigma-Aldrich

Poly(acrylic acid) solution, average M_w ~2,000, 50 wt. % in H₂O, electronic grade

181285

Sigma-Aldrich

Poly(acrylic acid), average M_v ~450,000