Skip to Content
Merck
  • Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Analytical chemistry (2015-03-17)
Evgeni Eltzov, Avital Cohen, Robert S Marks
ABSTRACT

Indoor air pollution became a recent concern found to be oftentimes worse than outdoor air quality. We developed a tool that is cheap and simple and enables continuous monitoring of air toxicity. It is a biosensor with both a nondisposable (monitor) and disposable (calcium alginate pads with immobilized bacteria) elements. Various parameters to enhance its signal have been tested (including the effect of the pad's orientation, it's exposure to either temperature or time with the air toxicant analyte, and various concentrations thereof). Lastly, the sensor has demonstrated its ability to sense the presence of chemicals in a real, indoor environment. This is the first step in the creation of a sensitive and simple operative tool that may be used in different indoor environments.

MATERIALS
Product Number
Brand
Product Description

Supelco
Diethylene glycol, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Acetone, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Formaldehyde-12C solution, 20% in H2O, 99.9 atom % 12C
Sigma-Aldrich
Chloroform, anhydrous, ≥99%, contains 0.5-1.0% ethanol as stabilizer
USP
Acetone, United States Pharmacopeia (USP) Reference Standard
USP
Diethylene glycol, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
(±)-Epichlorohydrin, purum, ≥99% (GC)
Supelco
Methanol, analytical standard
Supelco
Acetone, analytical standard
Sigma-Aldrich
Formaldehyde solution, Molecular Biology, 36.5-38% in H2O
Sigma-Aldrich
2-Mercaptoethanol, Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)
Sigma-Aldrich
Sodium chloride, Molecular Biology, DNase, RNase, and protease, none detected, ≥99% (titration)
Sigma-Aldrich
Sodium chloride, BioXtra, ≥99.5% (AT)
Sigma-Aldrich
Sodium chloride, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
Sigma-Aldrich
1-Octanol, ≥98%, FCC, FG
Sigma-Aldrich
Tetrahydrofuran, anhydrous, contains 250 ppm BHT as inhibitor, ≥99.9%
Sigma-Aldrich
N,N-Dimethylformamide, anhydrous, 99.8%
Sigma-Aldrich
Diethylene glycol, ReagentPlus®, 99%
Sigma-Aldrich
Acetone, suitable for HPLC, ≥99.9%
Sigma-Aldrich
Methanol, anhydrous, 99.8%
Sigma-Aldrich
Tetraethyl orthosilicate, ≥99.0% (GC)
Supelco
(±)-Epichlorohydrin, analytical standard
Sigma-Aldrich
2-Mercaptoethanol, BioUltra, Molecular Biology, ≥99.0% (GC)
Sigma-Aldrich
Diethylene glycol, puriss. p.a., ≥99.0% (GC), colorless
Sigma-Aldrich
Formaldehyde solution, Molecular Biology, BioReagent, ≥36.0% in H2O (T)
Sigma-Aldrich
Sodium chloride, BioUltra, Molecular Biology, ≥99.5% (AT)
Sigma-Aldrich
Formaldehyde solution, ACS reagent, 37 wt. % in H2O, contains 10-15% Methanol as stabilizer (to prevent polymerization)
Sigma-Aldrich
Tetrahydrofuran, anhydrous, ≥99.9%, inhibitor-free
Sigma-Aldrich
Piperidine, ReagentPlus®, 99%
Sigma-Aldrich
Formaldehyde solution, tested according to Ph. Eur.