Merck
All Photos(1)

Documents

F4135

Sigma-Aldrich

Fetal Bovine Serum

USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for insect cell culture, suitable for hybridoma

Sign Into View Organizational & Contract Pricing

Synonym(s):
HI FBS, HI FCS, HI sera, HI serum, FBS, FCS, sera, serum
MDL number:
eCl@ss:
32160409
NACRES:
NA.71

biological source

bovine fetus

sterility

sterile-filtered

product line

FBS Classic

composition

Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤20 mg/dL

origin

USA origin

technique(s)

cell culture | hybridoma: suitable
cell culture | insect: suitable
cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
F8192F0679F7942
vibrant-m

F4135

Fetal Bovine Serum

vibrant-m

F8192

Fetal Bovine Serum

vibrant-m

F0679

Fetal Bovine Serum

vibrant-m

F7942

Fetal Bovine Serum

origin

USA origin

origin

Australia origin

origin

non-USA origin

origin

Canada origin

biological source

bovine fetus

biological source

bovine fetus

biological source

bovine fetus

biological source

bovine fetus

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable, cell culture | insect: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

Quality Level

500

Quality Level

500

Quality Level

500

Quality Level

500

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

General description

Fetal Bovine Serum (FBS) remains a popular media supplement as it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and aids in proliferation. It also protects cells from oxidative damage and apoptosis. FBS blocks reactive oxygen species generation, which otherwise is not eliminated in serum-free media (SFM) systems.

Application

FBS sourced in the United States is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture.
Fetal Bovine Serum has been used:
  • as a component of Dulbecco′s Modified Eagles Media (DME, DMEM), Eagle′s Minimum Essential Medium (EMEM), Roswell Park Memorial Institute medium (RPMI-1640), α-modified eagle medium (αMEM) (MEM) and Leibovitz L-15 medium
  • as a component of blocking medium for treating brainstem slices
  • to improve attachment of dermal explants

Preparation Note

Prepared by heating at 56°C for 30 minutes.
This product is prepared by heating at 56°C for 30 minutes. Heating serum to 56°C for 30 minutes inactivates the complement system for immunoassays. Heat activation also inactivates other undetermined inhibitors of cell growth in culture.

Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 3

1 of 3

Fetal Bovine Serum Australia origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for hybridoma

Sigma-Aldrich

F8192

Fetal Bovine Serum

Gentamicin solution 50 mg/mL in deionized water, liquid, 0.1 μm filtered, BioReagent, suitable for cell culture

Sigma-Aldrich

G1397

Gentamicin solution

Combining probiotic Phaeobacter inhibens DSM17395 and broad-host-range vibriophage KVP40 against fish pathogenic vibrios
Rasmussen BB, et al
Aquaculture (Amsterdam, Netherlands), 513, 734415-734415 (2019)
Antileishmanial activity study and theoretical calculations for 4-amino-1, 2, 4-triazole derivatives
Suleymanouglu N, et al.
Journal of Molecular Structure, 1144, 80-86 (2017)
Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts: an in vitro approach to bovine digital dermatitis
Akkoc A, et al.
Journal of Applied Animal Research, 46, 445-450 (2018)
Deven Patel et al.
The Journal of biological chemistry, 291(17), 9322-9329 (2016-02-28)
During G1-phase of the cell cycle, normal cells respond first to growth factors that indicate that it is appropriate to divide and then later in G1 to the presence of nutrients that indicate sufficient raw material to generate two daughter
Ming Guan et al.
American journal of translational research, 11(3), 1343-1358 (2019-04-12)
Aging and exposure to noise or ototoxic drugs are major causes of hair cell death leading to human hearing loss, and many agents have been developed to protect hair cells from apoptosis. Fetal bovine serum (FBS) is a fundamental ingredient

Articles

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

Related Content

Learn how to effectively filter fetal bovine serum (FBS) using Stericup® Quick Release vacuum filtration devices, including flow test data and analysis.

Learn how to effectively filter fetal bovine serum (FBS) using Stericup® Quick Release vacuum filtration devices, including flow test data and analysis.

Learn how to effectively filter fetal bovine serum (FBS) using Stericup® Quick Release vacuum filtration devices, including flow test data and analysis.

Learn how to effectively filter fetal bovine serum (FBS) using Stericup® Quick Release vacuum filtration devices, including flow test data and analysis.

See All

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service