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QBD10200

Sigma-Aldrich

NHS-dPEG®4-biotin

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Synonym(s):
(+)-Biotin-PEO4-NHS-propionate, NHS-PEO4-Biotin
Empirical Formula (Hill Notation):
C25H40N4O10S
CAS Number:
Molecular Weight:
588.67
PubChem Substance ID:
NACRES:
NA.22

Assay

>90%

form

solid or viscous liquid

reaction suitability

reagent type: cross-linking reagent
reaction type: Biotinylations
reactivity: amine reactive

reagent type: linker

polymer architecture

shape: linear
functionality: monofunctional

shipped in

ambient

storage temp.

−20°C

SMILES string

O=C(ON1C(CCC1=O)=O)CCOCCOCCOCCOCCNC(CCCCC2C3NC(NC3CS2)=O)=O

InChI

1S/C25H40N4O10S/c30-20(4-2-1-3-19-24-18(17-40-19)27-25(34)28-24)26-8-10-36-12-14-38-16-15-37-13-11-35-9-7-23(33)39-29-21(31)5-6-22(29)32/h18-19,24H,1-17H2,(H,26,30)(H2,27,28,34)/t18-,19-,24-/m0/s1

InChI key

DTLVBHCSSNJCMJ-JXQFQVJHSA-N

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This Item
QBD10774QBD10198QBD10203
NHS-dPEG®4-biotin

QBD10200

NHS-dPEG®4-biotin

NHS-dPEG®24-biotin

QBD10774

NHS-dPEG®24-biotin

NHS-dPEG®12-biotin

QBD10198

NHS-dPEG®12-biotin

assay

>90%

assay

>90%

assay

>90%

assay

>90%

polymer architecture

shape: linear
functionality: monofunctional

polymer architecture

shape: linear
functionality: monofunctional

polymer architecture

shape: linear
functionality: monofunctional

polymer architecture

shape: linear
functionality: heterobifunctional

reaction suitability

reagent type: cross-linking reagent
reaction type: Biotinylations
reactivity: amine reactive, reagent type: linker

reaction suitability

-

reaction suitability

reagent type: cross-linking reagent
reaction type: Biotinylations
reactivity: amine reactive

reaction suitability

reagent type: cross-linking reagent
reaction type: Biotinylations
reactivity: amine reactive, reagent type: linker

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

General description

NHS-dPEG®4-biotin is a water-soluble cross-linking reagent used in biotinylation reactions. The N-hydroxysuccinimidyl (NHS) moiety of NHS-dPEG®4-biotin forms a stable amide bond with free primary amines, such as the amines on the side chain of lysine. The biotin moiety is used in a variety of applications, including labeling, supramolecular building, affinity chromatography, pull-down assays, and the production of biotinylated antibodies.

Features and Benefits

NHS-dPEG®4-biotin is used extensively to label molecules that contain a free amine with biotin. The single molecular weight NHS-dPEG®4-biotin has a long spacer of precise length (19.2 Å) between the biotin moiety and the labeled molecule. This dPEG® spacer imparts a high degree of water solubility to the biotin label. For biotinylated peptides and proteins, the dPEG® spacer dramatically reduces aggregation and precipitation of the modified molecule and reduces non-specific binding of the molecule, both of which are common problems with molecules that have been biotinylated with the widely used NHS-LC-biotin.

Legal Information

Products Protected under U.S. Patents # 7,888,536 B2
dPEG is a registered trademark of Quanta BioDesign

Storage Class Code

11 - Combustible Solids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Magnetic separation and enzymatic catalysis conjugated colorimetric immunosensor for Hepatitis B surface antigen detection
Hu Yongqin, et al.
Microchemical Journal, Devoted to the Application of Microtechniques in All Branches of Science, 168 (2021)

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