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Anti-Fas Antibody (human, neutralizing), clone ZB4

clone ZB4, Upstate®, from mouse

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APO-1 cell surface antigen, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, CD95 antigen, FASLG receptor, Fas (TNF receptor superfamily, member 6), Fas AMA, Fas antigen, apoptosis antigen 1, tumor necrosis factor receptor superfamily member 6, tu

biological source


Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies


ZB4, monoclonal

species reactivity





flow cytometry: suitable
neutralization: suitable
western blot: suitable



NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification


Gene Information

human ... FAS(355)

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This Item
biological source


biological source


biological source


biological source


antibody form

purified immunoglobulin

antibody form

affinity purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody


ZB4, monoclonal


CH11, monoclonal


2G3, monoclonal




flow cytometry: suitable, western blot: suitable, neutralization: suitable


flow cytometry: suitable, immunocytochemistry: suitable, western blot: suitable


indirect ELISA: suitable, proximity ligation assay: suitable, western blot: 1-5 μg/mL


western blot: 1:500-1:2000

Gene Information

human ... FAS(355)

Gene Information

human ... FAS(355)

Gene Information

human ... FAS(355)

Gene Information

human ... FAS(355)

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General description

Fas (also known as Apo-1 and CD95) is a 40-50 kDa cell surface glycoprotein and a member of the TNF receptor family. Fas is an apotosis signalling molecule that forms the death inducing signalling complex (DISC) upon binding by the Fas ligand trimer on an adjacent cell. The Fas receptor also trimerizes, after which it is internalized and can then bind to the adaptor molecule FADD. FADD facilitates binding of caspase-8. which is activated to induce caspase-8 mediated apoptosis. Fas also plays a role in T cell mediated cytotoxicity and T cell development. Fas is found in the thymus, liver, heart, and ovary, where it is expressed on a minority of resting T and B cells and monocytes. The antagonistic ZB4 clone anti-Fas monoclonal has been used to block the apoptosis-inducing activity of the Anti-Fas CH-11 clone in multiple publications.
This product does not induce apoptosis in cell culture.


This antibody does not recognize TNF and mouse Fas.
This antibody recognizes the human cell surface antigen Fas expressed in various human cells, including myeloid cells, T lympho-blastoid cells and diploid fibroblasts. It does not induce apoptosis in cell culture.


Recombinant human Fas.


Anti-Fas Antibody (human, neutralizing), clone ZB4 detects level of Fas & has been published & validated for use in WB, FC, NEUT.
Flow Cytometry: A previous lot of this antibody used 5-20 μg/mL. Western Blot: A previous lot of this antibody used 5 μg/mL.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional


Evaluated by neutralization of apoptosis induced by anti-Fas (human, activating), clone CH11 (Catalog # 05-201) treatment of human Jurkat cells.

Neutralization: 10-500 ng/mL of this antibody was incubated for one hour with human Fas-transfected cells and inhibited the anti-Fas (human, activating), clone CH11 (Catalog # 05-201). Cell viability was assessed using a WST-1 assay

Target description

45 kDa

Physical form

Ammonium sulfate and purified mouse monoclonal IgG1 in PBS containing 50% glycerol. Store at -20°C.
Format: Purified
Protein A purified

Storage and Stability

Stable for 1 year at from date of receipt.

Analysis Note

Various human cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids



Certificates of Analysis (COA)

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Stimulated human lamina propria T cells manifest enhanced Fas-mediated apoptosis
Boirivant, M., et al
The Journal of Clinical Investigation, 98, 2616-2622 (1996)
Networked T cell death following macrophage infection by Mycobacterium tuberculosis.
Macdonald, SH; Woodward, E; Coleman, MM; Dorris, ER; Nadarajan, P; Chew, WM; McLaughlin et al.
Testing null
E Lacana et al.
Nature medicine, 5(5), 542-547 (1999-05-06)
Programmed cell death is a process required for the normal development of an organism. One of the best understood apoptotic pathways occurs in T lymphocytes and is mediated by Fas/Fas ligand (FasL) interaction. During studies of apoptosis induced by T
Divya Kesanakurti et al.
PloS one, 6(5), e19341-e19341 (2011-05-17)
Abrogation of apoptosis for prolonged cell survival is essential in cancer progression. In our previous studies, we showed the MMP-2 downregulation induced apoptosis in cancer cell lines. Here, we attempt to investigate the exact molecular mechanism of how MMP-2 depletion
Bin Zhang et al.
The Journal of biological chemistry, 278(31), 28443-28454 (2003-05-09)
Phagocytosis of complement-opsonized targets is a primary function of neutrophils at sites of inflammation, and the clearance of neutrophils that have phagocytosed microbes is important for the resolution of inflammation. Our previous work suggests that phagocytosis leads to rapid neutrophil

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