05-591

Anti-Akt/PKB Antibody, PH Domain, clone SKB1

clone SKB1, Upstate^®, from mouse

• Synonym(s): Anti-AKT, Anti-PKB, Anti-PKB-ALPHA, Anti-PRKBA, Anti-RAC, Anti-RAC-ALPHA
• eCl@ss: 32160702

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: SKB1, monoclonal
• species reactivity: rat, human, mouse
• packaging: antibody small pack of 25 μg
• manufacturer/tradename: Upstate^®
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_005163
• UniProt accession no.: P31749
• shipped in: dry ice
• Gene Information: mouse ... Akt1(11651)

Description

General description

Akt (protein kinase B), a serine/threonine kinase, has emerged as a critical enzyme in signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. In mammals three isoforms of Akt (α, β, γ or Akt 1, 2, 3) are reported that exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt α is the predominant isoform in most tissues, whereas the highest expression of Akt β is observed in the insulin-responsive tissues, and Akt γ is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal pleckstrin homology (PH) domain that provides a lipid-binding module to direct Akt to PIP2 and PIP3, a central catalytic domain, and a C-terminal hydrophobic motif.

Application

Detect Akt/PKB using this Anti-Akt/PKB Antibody, PH Domain, clone SKB1 validated for use in FC, IC, IP & WB.

Quality

routinely evaluated by immunoblot on RIPA lysates from A431 cells

Target description

60 kDa

Physical form

Format: Purified

Protein G Purified immunoglobulin in Immunoaffinity Purified immunoglobulin in 0.02 M phosphate buffer, pH 7.6, 0.25M NaCl, 0.1% sodium azide and 30% glycerol.

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany