Merck
All Photos(6)

06-680

Sigma-Aldrich

Anti-Androgen Receptor Antibody

from rabbit, purified by affinity chromatography

Synonym(s):
Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, human

species reactivity (predicted by homology)

bovine (based on 100% sequence homology), canine (based on 100% sequence homology), horse (based on 100% sequence homology), mouse (based on 100% sequence homology)

technique(s)

ChIP: suitable (ChIP-seq)
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

bovine ... Ar(280675)
dog ... Ar(403588)
human ... AR(367)
mouse ... Ar(11835)
rat ... Ar(24208)

General description

The androgen receptor (AR) is a type of nuclear receptor which is activated by binding of either of the androgenic hormones testosterone or dihydrotestosterone. The main function of the androgen receptor is as a DNA binding transcription factor which regulates gene expression. However the androgen receptor also has additional functions independent of DNA binding. The androgen receptor is most closely related to the progesterone receptor, and progestins in higher dosages can block the androgen receptor.

Immunogen

Epitope: This antibody recognizes the Modulation Region within the N-terminus of Androgen Receptor.
KLH-conjugated linear peptide corresponding to the N-terminus of rat Androgen Receptor.

Application

Anti-Androgen Receptor Antibody, PG-21 is a Rabbit Polyclonal Antibody for detection of Androgen Receptor also known as Dihydrotestosterone Receptor & has been tested in ICC, IHC, IP & WB.
Immunoprecipitation Analysis: 34 µL from a representative lot immunoprecipitated Androgen Receptor from 1000 µg of LNCaP cell lysate.

Flow Cytometry Analysis: A 1:300 dilution from a representative lot detected Androgen Receptor in 0.2X10E6 MCF-7 cells.

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Androgen Receptor from DU145 cells stably transfected with a 7 kD-PSA promoter, different AR poly A lentiviral vectors, and a Flag-tagged hZMIZ1 vector (Li, X., et al. (2011). PLoS One. 6(9):e25040.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Androgen Receptor in embryonic rat tissue, neonatal rat testis tissue, and adult rat testis tissue (Nation, T., et al. (2010). J Pediatr Surg. 46(8): 1539-1543.).

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Androgen Receptor in MCF-7 cells stabily transfected with Androgen Receptor (Ghatge, R. P., et al. (2005). Breast Cancer Res. 7(6):R1036-1050.).

Chromatin Immunoprecipitaton-Sequence Analysis: A representative lot from an independent laboratory immunoprecipitated Androgen Receptor from LNCaP cell lysates under certain treated conditions (Zhao, J. C., et al. (2012). Genome Res. 22(2):322-331.).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

Quality

Evaluated by Western Blotting in LNCaP cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Androgen Receptor in 10 µg of LNCaP cell lysate.

Target description

~110 kDa observed

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service