118502

InSolution ATM Kinase Inhibitor

The InSolution ATM Kinase Inhibitor controls the biological activity of ATM Kinase. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.

• Synonym(s): InSolution ATM Kinase Inhibitor
• Empirical Formula (Hill Notation): C₂₁H₁₇NO₃S₂
• Molecular Weight: 395.49

Properties

• Quality Level: 100
• Assay: ≥95% (HPLC)
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic); protect from light
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

A cell-permeable disubstituted pyranone compound that acts as a potent and ATP-competitive inhibitor of ATM kinase (IC₅₀ = 13 nM; K_i = 2.2 nM). Displays excellent selectivity over other PIKK family kinases (IC₅₀ = 2.5, 9.3, 16.6 µM for DNA-PK, mTOR, PI 3-K, respectively; IC₅₀ >100 µM for PI 4-K and ATR) and exhibits little activity towards a panel of 60 other kinases even at concentrations as high as 10 µM. Inhibits ATM-dependent cellular protein phosphorylation following ionizing radiation (IR) and sensitizes cells with wild-type ATM, but not mutant ATM, to the cytotoxic effects of IR and DNA-damaging agents.

Packaging

Packaged under inert gas

Warning

Toxicity: Irritant (B)

Physical form

A 10 mM (2 mg/506 µl) solution of ATM Kinase Inhibitor (Cat. No. 118500) in DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Pereg, Y., et al. 2005. Proc. Natl. Acad. Sci. USA102, 5056.
Lau, A., et al. 2005. Nat. Cell Biol.7, 493.
Hickson, I., et al. 2004. Cancer Res.64, 9152.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany