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Millipore

HEIMPLATE XLD Agar - LI EP+USP 30ml

Selective media for the isolation of Salmonella and Shigella in pharmaceuticals and clinical materials

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Synonym(s):
Xylose Lysine Deoxycholate Agar

Agency

EP 2.6.12
USP 61

Quality Level

sterility

sterile

form

solid

feature

ready-to-use

technique(s)

microbiological culture: suitable

pH

7.4 ( in H2O)

application(s)

microbiology
pharmaceutical

storage temp.

20-25°C

suitability

Salmonella spp. (spp.)

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This Item
1.467511.052871.00215
Readyplate XLD Agar acc ISO 6579 For isolation and differentiation of Salmonella, box of 20 plates, plate diam. 90 mm

1.46751

Readyplate XLD Agar acc ISO 6579

agency

EP 2.6.12, USP 61

agency

APHA, ISO 19250, ISO 6579

agency

APHA, ISO 19250, ISO 6579

agency

FDA-BAM, according to ISO 21567, according to ISO 6579

form

solid

form

solid

form

medium granules (dehydrated (DCM))

form

granular (dehydrated culture medium)

technique(s)

microbiological culture: suitable

technique(s)

-

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

sterility

sterile

sterility

-

sterility

-

sterility

-

application(s)

microbiology
pharmaceutical

application(s)

food and beverages
industrial qc
microbiology

application(s)

food and beverages, microbiology

application(s)

clinical
food and beverages
industrial qc
pharmaceutical, microbiology

General description

Xylose Lysine Deoxycholate agar (XLD agar) is a selective growth medium introduced by Taylor (1965) for the isolation of Salmonella and Shigella species (Taylor and schelbert, 1971). It relies on the use of selective inhibitory component sodium deoxycholate and three indicator systems, i.e. xylose, lactose and sucrose combined with phenol red. Degradation of xylose, lactose, and sucrose to acid causes phenol red to change its colour to yellow. Sodium thiosulfate and iron (III) salt react to form a precipitate of black iron sulfide in the colonies, indicating the production of hydrogen sulfide. Bacteria which decarboxylate lysine to cadaverine can be recognized by the appearance of a purple coloration around the colonies due to an increase in pH. These reactions can proceed simultaneously or successively. This causes the pH indicator to exhibit various shades of colour, or it may change its colour from yellow to red on prolonged incubation. The culture medium is weakly inhibitory to other microbes due to the presence of sodium deoxycholate.

Application

Xylose Lysine Deoxycholate (XLD) Agar - LI is a selective culture medium for isolation of Salmonella and Shigella in pharmaceuticals.

Packaging

90 mm settle plates with 30 ml filling volume (20 or 120 plates per box) for long incubation (LI)

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 2


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Handbook of culture media for food and water microbiology
Curtis, GDW and Baird, Rosamund M and Corry, Janet EL et al.
Royal Society of Chemistry (2011)

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