Merck
All Photos(2)

16-256

Sigma-Aldrich

Anti-Phosphatidylserine Antibody, clone 1H6, Alexa Fluor® 488

clone 1H6, Upstate®, from mouse

eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

ALEXA FLUOR® 488

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1H6, monoclonal

species reactivity

vertebrates

manufacturer/tradename

Upstate®

technique(s)

flow cytometry: suitable

isotype

IgG

shipped in

wet ice

General description

Significance:
Anti-Phosphatidylserine may be used to detect translocation of the membrane phospholipid phosphatidylserine (PS) from the inner to the outer cell membrane leaflet; it provides an alternative to Annexin V for quantitation of apoptosis.
Phosphatidylserine, or PS, is a naturally occurring, phospholipid nutrient. PS is essential to the functioning of all the cells of the body, but is most concentrated in the brain. Its relative abundance in this organ reflects its proven involvement in an assortment of nerve cell functions, including nerve transmitter release and synaptic activity. Clinical studies have suggested that PS can support brain functions that tend to decline with age.

Specificity

Recognizes phosphatidylserine (PS) in cell membranes.

Immunogen

Liposomes containing 70% phosphatidylserine and 30% phosphatidylglycerol.

Application

Detect Phosphatidylserine using this Anti-Phosphatidylserine Antibody, clone 1H6, Alexa Fluor 488 validated for use in FC.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Quality

Flow Cytometry: 0.2 μg of this antibody detected Phosphatidylserine in fixed Jurkat cells (see page 2).

Apoptosis Assay: Time course for induction of apoptosis in Jurkat cells by staurosporine, measured using either an Anti-Phosphatidylserine, clone 1H6, Alexa Fluor® 488 conjugate or an Annexin V FITC conjugate.

Physical form

Protein G Purified
Purified mouse monoclonal IgG in buffer containing PBS containing 1% BSA, 0.05% Tween, 0.05% sodium azide. Liquid at 4ºC.

Storage and Stability

Stable for 1 year at from date of receipt.

Analysis Note

Control
Staurosporine treated Jurkat cells.
Included Negative Control: Catalog # 16-240, Alexa Fluor® 488-conjugated Normal Mouse IgG.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service