203195

Protein A, Biotin Conjugate

This Protein A, Biotin Conjugate is validated for use in Solid Phase Immunoassay.

Properties

• Quality Level: 100
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Native protein from Straphylococcus Aureus, conjugated to biotin. Recognizes the Fc portions of immunoglobulins of various species.

Native protein A conjugated to biotin.

Application

Solid Phase Immunoassay (1:10,000)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 10 mM PBS, 1% BSA, pH 7.6.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

A single band at MW 50,000 by SDS-PAGE

Other Notes

The protein A: biotin molar ratio is determined on a lot-specific basis. Binding strength is as follows: strongly reactive, guinea pig, human IgG1, IgG2, and IgG4, and rabbit; weakly reactive: bovine, human IgA, IgM, and IgE, and mouse; nonreactive: goat, horse, human IgD and IgG3, rat, and sheep. o-phenylenediamine, 1.0 mg/ml in citrate buffer, pH 4.5, 0.1% urea peroxide at 25°C, was used as substrate for testing biotin-Protein A and avidin peroxidase functionality. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable