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5.30650

Sigma-Aldrich

Luciferase Substrate, CycLuc1

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Synonym(s):
Luciferase Substrate, CycLuc1
Empirical Formula (Hill Notation):
C13H11N3O2S2
Molecular Weight:
305.38

Assay

≥94% (HPLC)

Quality Level

form

powder

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

yellow

solubility

DMSO: 100 mg/mL

storage temp.

2-8°C

General description

A synthetic cyclic alkylaminoluciferin that exhibits properties that are superior to those of D-luciferin. Binds to luciferase with higher affinity and and emits about 5.7-fold more light than aminoluciferin and 3.2-fold more light than D-luciferin. Its superior light output is maintained over a wide range (1-100 µM). Shown to readily cross the blood-brain barrier in mice and provides much improved noninvasive bioluminescence imaging (BLI) of the brain at lower dose when compared to D-luciferin. Yields about 10-fold higher bioluminescent signal than D-luciferin when Luc2 luciferase expressing 4T1 breast cancer cells are implanted into the mammary fat pads of BABL/c mice. A rapid (4-5 min), long-lasting, and steady signal peak is observed following an i.v injection CycLuc1 in mice.
A synthetic cyclic alkylaminoluciferin that exhibits properties that are superior to those of D-luciferin. Binds to luciferase with higher affinity and and emits about 5.7-fold more light than aminoluciferin and 3.2-fold more light than D-luciferin. Its superior light output is maintained over a wide range (1-100 µM). Shown to readily cross the blood-brain barrier in mice and provides much improved noninvasive bioluminescence imaging (BLI) of the brain at lower dose when compared to D-luciferin. Yields about 10-fold higher bioluminescent signal than D-luciferin when Luc2 luciferase expressing 4T1 breast cancer cells are implanted into the mammary fat pads of BABL/c mice. A rapid (4-5 min), long-lasting, and steady signal peak is observed following an i.v injection CycLuc1 in mice.

Please note that the molecular weight for this compound is batch-specific due to variable water content.

Biochem/physiol Actions

Cell permeable: yes
Primary Target
Luciferase

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Other Notes

Evans, M., et al. 2014. Nat Methods.11, 393.
Reddy, G. R, et al. 2010. J. Am. Chem. Soc.132, 13586.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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