556746

RNase A, Protease-Free, Highly Purified, Bovine Pancreas

For selective degradation of RNA

• Synonym(s): RNase A, Protease-Free, Highly Purified, Bovine Pancreas, Ribonuclease A
• CAS Number: 9001-99-4
• Enzyme Commission number: 3.1.4.22 (BRENDA, IUBMB)

Properties

• Quality Level: 100
• assay: ≥95% (SDS-PAGE)
• form: lyophilized solid
• specific activity: 81-99 units/mg material
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated
• solubility: water: 2 mg/mL
• foreign activity: Protease, none detected
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Note: 1 KU = 1000 units.

Ribonuclease A, Protease-Free is a

chromatographically purified, pyrimidine-specific

endoribonuclease that acts on single-stranded

RNA.

Ribonuclease A, Protease-Free is achromatographically purified, pyrimidine-specificendoribonuclease that acts on single-strandedRNA.

Packaging

50 ku in Glass bottle

10 ku in Plastic ampoule

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will catalyze the hydrolysis of RNA to yield a first-order velocity constant equal to 1 at 25°C, pH 5.0.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Analysis Note

May contain trace amounts of DNase I, which can be inactivated by boiling for 15 min in 50 mM potassium acetate, pH 5.5.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable