ABC929

Anti-MAP1LC3A antibody

rabbit polyclonal

• Synonym(s): Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated protein 1 light chain 3 alpha
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: polyclonal
• Species reactivity: human
• Application: ICC, WB
• Citations: 33

Properties

• Product Name: Anti-LC3-I/II Antibody, from rabbit, purified by affinity chromatography
• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): Xenopus (based on 100% sequence homology), rat (based on 100% sequence homology), porcine (based on 100% sequence homology), mouse (based on 100% sequence homology), zebrafish (based on 100% sequence homology), bovine (based on 100% sequence homology)
• technique(s): immunocytochemistry: suitable, western blot: suitable
• NCBI accession no.: NP_115903
• UniProt accession no.: Q9H492
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... MAP1A(4130), MAP1LC3A(84557)

Description

General description

Microtubule-associated LC3A constitute nearly half of the mass of all the microtubule associated proteins that copurify with brain microtubules. LC3A is composed of a heavy chain and multiple light-chain subunits. The molecular machinery required for autophagy is highly conserved in all eukaryotes as seen by the high degree of conservation of proteins involved in the formation of the autophagosome membranes. Recently, both yeast Apg8p and its rat homologue LC3-II were identified as essential constituents of autophagosome membrane as a processed form. In addition, both the yeast and human proteins exist in two modified forms produced by a series of post-translational modifications including a critical C-terminal cleavage after a conserved Gly residue, and the smaller processed form is associated with the autophagosome membranes Results revealed different regulation of the three human isoforms of LC3-II and implicate that the three isoforms may have different physiological functions. Two forms of LC3A, called LC3-I and -II, were produced post-translationally in various cells. LC3-I is cytosolic, whereas LC3-II is membrane bound. LC3-II is the first mammalian protein identified that specifically associates with autophagosome membranes.

~17/14 kDa observed

Immunogen

Recombinant protein corresponding to Human LC3-I/II.

Application

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected LC3 in serum-starved, chloroquine-treated HeLa cells. A punctate distribution is seen, evidence of autophagy conditions within the cell.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-LC3-I/II Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of LC3-I/II.

Biochem/physiol Actions

This antibody detects both the non-lipidated LC3-I (non-autophagic) and lipidated LC3-II (autophagic) under autophagy conditions.

Physical form

Antigen Affinity Purified

Purified rabbit Polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in serum-starved, chloroquine-treated HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected LC3-I (non-lipidated) and LC3-II (lipidated) in 10 µg of serum-starved, chloroquine-treated HeLa cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Replaces: MABC177; MABC176

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable