Acetyl-CoA carboxylase 1 (UniProt: Q13085; also known as EC: 18.104.22.168, ACC1, ACC-alpha) is encoded by the ACACA (also known as ACAC, ACC1, ACCA) gene (Gene ID: 31) in human. ACC1 is a cytoplasmic protein that is expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues. It is also expressed at low level in pulmonary tissue, but is not detected in the liver. ACC1 can be present as a monomer, homodimer, or homotetramer. It can also form filamentous polymers. Four isoforms of ACC1 have been described that are produced by alternative splicing. It catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids and carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase. The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. ACC1 levels are regulated under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Mutations in ACACA gene can result in ACC1 deficiency and lead to error of de novo fatty acid synthesis associated with severe brain damage, persistent myopathy and poor growth.
This rabbit polyclonal antibody detects Acetyl-CoA carboxylase 1 in human cells. It targets an epitope within 245 amino acids from the C-terminal region.
GST-tagged recombinant fragment corresponding to 245 amino acids from the C-terminal half of human Acetyl-CoA carboxylase 1.
Anti-Acetyl CoA Carboxylase 1, Cat. No. ABS1068, is a rabbit polyclonal antibody that detects Acetyl CoA Carboxylase 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Acetyl CoA Carboxylase 1 in human liver and human kidney tissue sections.
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Acetyl CoA Carboxylase 1 in A431 cell lysate.
~220 kDa observed; 265.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
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