Sterol regulatory element-binding protein 1 (UniProt: P36956; also known as SREBP-1, Class D basic helix-loop-helix protein 1, bHLHd1, Sterol regulatory element-binding transcription factor 1) is encoded by the SREBF1 (also known as BHLHD1, SREBP1) gene (Gene ID: 6720) in human. SREBP-1 is a multi-pass membrane protein that acts as at transcriptional activator required for lipid homeostasis. Six isoforms of SREBP-1 have been described that are produced by alternative splicing. SREBP-1 is expressed in a wide variety of tissues and is most abundant in liver and adrenal gland. In fetal lung and liver it is expressed in high levels. SREBP-1A predominates in hepatoma cell lines while isoform SREBP-1C is predominantly found in liver, adrenal gland, and ovary. Both isoforms IA and IC is found in kidney, brain, white fat, and muscle. SREBP-1 regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. It binds to the sterol regulatory element 1 (SRE-1) (5′-ATCACCCCAC-3′) and has dual sequence specificity binding to both an E-box motif (5′-ATCACGTGA-3′) and to SRE-1 (5′-ATCACCCCAC-3′). SREBP-1 has two cytoplasmic domains (aa 1-487 and 567-1147; two helical domains (aa 488-508 and 548-568), and a luminal domain (aa 509-547). In the absence of sterols, it moves from the endoplasmic reticulum to the Golgi. At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane.
This rabbit polyclonal antibody detects Sterol regulatory element-binding protein 1 in human.
KLH-conjugated linenar peptide corresponding to 14 amino acids from the N-terminal half of human Sterol regulatory element-binding protein 1. The Immunogen sequence is conserved in SREBP-1A, B, and C and in isoform 4.
Anti-SREBP-1, Cat. No. ABS1508, is a rabbit polyclonal antibdody that detects Sterol regulatory element-binding protein 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected SREBP-1 in human adrenal gland and human kidney tissues.
Evaluated by Western Blotting in DU145 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected SREBP-1 in 10 µg of DU145 cell lysate.
~120 kDa observed; 121.67 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
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