Merck
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GF102

Sigma-Aldrich

TWEAK, human

Human TWEAK is a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis.

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eCl@ss:
32160405

biological source

human

Quality Level

Assay

>98% (SDS-PAGE and HPLC)

manufacturer/tradename

Chemicon®

technique(s)

cell culture | mammalian: suitable

input

sample type mesenchymal stem cell(s)

NCBI accession no.

UniProt accession no.

shipped in

dry ice

Gene Information

human ... TNFSF12(8742)

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This Item
GF348GF092SRP3106
Sigma-Aldrich

Sigma-Aldrich

GF102

TWEAK, human

Sigma-Aldrich

Sigma-Aldrich

SRP3106

LIGHT human

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

-

biological source

human

biological source

-

biological source

human

biological source

human

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

input

sample type mesenchymal stem cell(s)

input

-

input

sample type hematopoietic stem cell(s)

input

-

General description

Human TWEAK is a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis. TWEAK, like many members of the TNF family, was able to induce interleukin-8 synthesis in a number of cell lines. GF102 is a 17.0 kDa protein, comprising the TNF-like extracellular domain of TWEAK.
Product Source: E.coli

Physical form

Sterile filtered and then the protein was lyophilized from 10 mM NaP pH 7.5 + 0.1 M L-Arg.

Storage and Stability

The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C. Freeze/thaw cycles will result in significant loss of activity. After a quick spin, reconstitute in 10 mM NaP pH 7.5 to a concentration of 1 mg/ml. This solution can be diluted into other buffer solutions or stored at -20°C. Reconstituted TWEAK should be stored at -20°C or lyophilized in working aliquots.

Analysis Note

Specific Activity: GF102 is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of IL-8 production by Human PBMC is less than 10ng/ml.

GF102 weakly induces the death of HT29 cells when cultured in the presence of IFN-g. The ED50 for this effect is between 30-45 ng/ml.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1


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