Merck
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GF314

Sigma-Aldrich

TNF- α Protein, Human Recombinant Animal Free

Tumor necrosis factor alpha (TNF-α), also known as cachectin & TNFSF1A, is the prototypic ligand of the TNF superfamily. Recombinant animal free human TNF-α is manufactured using all non-animal reagents.

Synonym(s):
Tumor Necrosis Factor Alpha, TNFa, TNF-alpha, Cachectin, TNFSF1A
eCl@ss:
32160405

recombinant

expressed in E. coli

Quality Level

Assay

>98% (SDS-PAGE and HPLC)

impurities

<0.1 μg/mg protein endotoxin (<1EU/μg)

NCBI accession no.

UniProt accession no.

shipped in

dry ice

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T5944GF023SRP2102
recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

-

recombinant

expressed in E. coli

assay

>98% (SDS-PAGE and HPLC)

assay

≥98% (SDS-PAGE and HPLC)

assay

>97% (SDS-PAGE)

assay

≥80% (SDS-PAGE)

impurities

<0.1 μg/mg protein endotoxin (<1EU/μg)

impurities

<1 EU/μgtested (LAL test)

impurities

-

impurities

-

NCBI accession no.

NM_000594.2

NCBI accession no.

-

NCBI accession no.

NM_000594.2

NCBI accession no.

NM_000594

UniProt accession no.

P01375

UniProt accession no.

P16599

UniProt accession no.

P01375

UniProt accession no.

P01375

shipped in

dry ice

shipped in

-

shipped in

dry ice

shipped in

dry ice

General description

Product Source: E. coli
Tumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF1A, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-α is produced by a wide variety of immune and epithelial cell types. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes. Recombinant human TNF-α is a soluble 157 amino acid protein (17.4 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.

Specificity

Cross Reactivty
Cow, Monkey, Mouse, Rat, Sheep

Application

Research Category
Inflammation & Immunology
Research Sub Category
Growth Factors & Receptors

Quality

Bioactivity assay: The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomyocin D is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥2 x 107 units/mg.

Physical form

Product is filtered through a 0.2 micron filter before lyophilization.
Recombinant animal free human TNF- α is manufactured using all non-animal reagents

Storage and Stability

Store at -20°C for up to 6 months from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


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