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IF009

Sigma-Aldrich

Interferon-α A Protein, Recombinant mouse

Interferon-α A Protein, Recombinant mouse.

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Synonym(s):
Interferon alpha-2
eCl@ss:
32160405
NACRES:
NA.75

biological source

mouse

Quality Level

recombinant

expressed in E. coli

Assay

>95%

form

liquid

manufacturer/tradename

Chemicon®

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

shipped in

dry ice

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I8657I9032I4276
Sigma-Aldrich

Sigma-Aldrich

I8657

Interferon α from rat

Interferon-αA human ≥95%, suitable for cell culture, buffered aqueous solution, recombinant, expressed in E. coli

Sigma-Aldrich

I4276

Interferon-αA human

recombinant

expressed in E. coli

recombinant

expressed in bacteria

recombinant

expressed in E. coli

recombinant

expressed in E. coli

assay

>95%

assay

≥95%

assay

≥95%

assay

≥95%

form

liquid

form

lyophilized powder

form

buffered aqueous solution

form

buffered aqueous solution

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

P01563, P01573

UniProt accession no.

P05011

UniProt accession no.

P01575

UniProt accession no.

P01563

General description

Product Source: Gene obtained from mouse DNA expressed in E. coli.
Purified Recombinant Mouse Interferon Alpha A (IFNalphaA)

Unit Definition

Specific Activity: The units are determined with respect to the international reference standard for mouse interferon alpha (IFNalpha) provided by the National Institutes of Health.Unit of activity measured on mouse L929 cells with encephalomyocarditis virus (EMCV).

Physical form

Frozen, in PBS containing 0.1% bovine serum albumin (BSA).

Storage and Stability

After receipt, this product should be kept at -70°C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Use polypropylene pipette tips and tubes when diluting this interferon. Dilute only in buffers containing protein such as 0.1% bovine serum albumin.

Analysis Note

Specific Activity: Determined by assaying inhibition of virus-induced cytopathic effect.In this antiviral assay for interferon, about 1 unit/mL of interferon is the quantity necessary to produce a cytopathic effect of 50%

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Tingting Deng et al.
Immunology and cell biology, 91(1), 52-59 (2012-09-19)
Toll-like receptor (TLR) activation by microbial pathogens triggers inflammatory responses against microbes. The phagocytic clearance of invading microbes and apoptotic immune cells is essential to resolve inflammation. However, the relationship between TLR activation and phagocytosis is poorly understood. We found
Jon B Suzich et al.
EMBO reports, 22(9), e52547-e52547 (2021-07-02)
Herpes simplex virus (HSV) establishes latent infection in long-lived neurons. During initial infection, neurons are exposed to multiple inflammatory cytokines but the effects of immune signaling on the nature of HSV latency are unknown. We show that initial infection of

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