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MAB1397Z

Sigma-Aldrich

Anti-ICAM-1 Antibody, clone 3E2B, Azide Free

clone 3E2B, Chemicon®, from hamster(Armenian)

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Synonym(s):
CD54, MAB1397
eCl@ss:
32160702
NACRES:
NA.41

biological source

hamster (Armenian)

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

3E2B, monoclonal

species reactivity

mouse

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

mouse ... Icam1(15894)

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This Item
MAB2146ZMAB2146WH0003383M1
antibody form

affinity purified immunoglobulin

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified immunoglobulin

clone

3E2B, monoclonal

clone

P2A4, monoclonal

clone

P2A4, monoclonal

clone

3H8-2G6, monoclonal

species reactivity

mouse

species reactivity

human

species reactivity

human

species reactivity

human

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

technique(s)

ELISA: suitable, flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable

technique(s)

ELISA: suitable, flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable

technique(s)

ELISA: suitable, flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable

technique(s)

immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 1-5 μg/mL

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Specificity

MAB1371Z is reactive with mouse ICAM-1 (CD54), which is present on T-cells, B-cells, monocytes and endothelial cells, epithelial cells, dendritic cells and keratinocytes. Expression of CD54 is increased after stimulation with cytokines and LPS, and it also plays a role in B cell-T cell interactions.

Application

Anti-ICAM-1 Antibody, clone 3E2B, Azide Free detects level of ICAM-1 & has been published & validated for use in ELISA, FC, FUNC, IC, IH.
Immunocytochemistry: Live or acetone fixed cells

ELISA

Immunohistochemistry of acetone fixed frozen sections. (Paraffin not tested).

Flow Cytometry: Suggested working dilution 50 μg/mL. Use 5 mL of this dilution to label 0.5 x 10(e6) cells.

Immunoprecipitation: use protein G or rabbit anti-hamster IgG secondary

Clone 3E2B is reported to be function blocking against murine ICAM-1.

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)

Linkage

Replaces: CBL1331F

Physical form

Format: Purified
Purified IgG prepared by affinity chromatography on Protein G agarose beads. Liquid at 1 mg/mL in sterile-filtered PBS, containing no preservatives. Endotoxin level 4.13EU/mg

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze-thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Dominique Promeneur et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(3), 1035-1040 (2013-01-02)
Tragically common among children in sub-Saharan Africa, cerebral malaria is characterized by rapid progression to coma and death. In this study, we used a model of cerebral malaria appearing in C57BL/6 WT mice after infection with the rodent malaria parasite

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