Calretinin is a 29 kDa calcium binding protein that is expressed in central and peripheral nervous system and in many normal and pathological tissues. Calretinin is also considered an important diagnostic aid in the differential diagnosis of cystic and solid ameloblastic tumors. The rat and human calretinin exhibit 98% sequence homology and 91% homology to many other species. Calretinin is generally expressed with two other calcium binding proteins Calbindin D-29 and parvalbumin in CNS and other peripheral tissues. Calretinin antibodies have been used to detect a number of carcinomas by clinicopathological, immunohistochemical techniques including, epithelial malignant mesotheliomas, Ovarian yolk sac tumor, Adenomatoid tumors of the uterus etc
Calretinin is a neuron specific calcium binding protein (31 kDa) found mostly in the CNS and retina. It is present in subsets of neurons throughout the brain and spinal cord, including sensory ganglia. It shares about 55% amino acid homology with calbindin, another calcium binding protein found in brain and periphery. Studies suggest that calretinin, like calbindin, may be neuroprotective.
Other species have not been tested.
Recombinant rat calretinin (Heinze, L., 2007).
Anti-Calretinin Antibody, clone 6B8.2 detects level of Calretinin & has been published & validated for use in IH & WB.
On rat brain tissue:
The antibody has been used successfully on formalin-fixed, paraffin embedded human tissue after pre-treatment of the tissue slides with citrate buffer and heat treatment. Suggested starting dilution 1:100-1:400. Proteolytic digestion with pepsin is detrimental to the immunostaining.
Optimal working dilutions must be determined by the end user.
Research Sub Category
Routinely evaluated by Western Blot on mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected Calretinin on 10 μg of mouse brain lysates.
Protein A purified
Purified mouse monoclonal IgG1 in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.
Storage and Stability
Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
Rat brain cortex, Mesothelioma tissue, mouse brain lysate.
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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