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MAB2146Z

Sigma-Aldrich

Anti-ICAM-1 Antibody, clone P2A4, Azide Free

clone P2A4, Chemicon®, from mouse

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Synonym(s):
CD54, MAB2146
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

P2A4, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... ICAM1(3383)

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MAB2146SAB1405977SAB4700329
vibrant-m

MAB2146

Anti-ICAM-1 Antibody, clone P2A4

Gene Information

human ... ICAM1(3383)

Gene Information

human ... ICAM1(3383)

Gene Information

human ... ICAM1(3383)

Gene Information

human ... ICAM1(3383)

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

clone

P2A4, monoclonal

clone

P2A4, monoclonal

clone

polyclonal

clone

1H4, monoclonal

technique(s)

ELISA: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, flow cytometry: suitable, immunohistochemistry: suitable

technique(s)

ELISA: suitable, flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable

technique(s)

western blot: 1 μg/mL

technique(s)

flow cytometry: suitable

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Specificity

Human ICAM-1

Immunogen

IL-1beta activated HUVEC (human)

Application

Immunohistochemistry: for use on acetone or paraformaldehyde fixed tissue

Immunocytochemistry: for use on B cells, PBL, T cells

Immunoprecipitation

FACS

ELISA

Inhibits ICAM-1 dependent adhesive interactions

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)
This Anti-ICAM-1 Antibody, clone P2A4, Azide Free is validated for use in ELISA, FC, IP, IC, IH, FUNC for the detection of ICAM-1.

Physical form

Format: Purified
Sterile-filtered lquid containing 0.2M phosphate buffer, 0.25M sodium chloride, no preservatives.

Storage and Stability

Maintain at -20°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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T1503
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25G
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Kelly M Bailey et al.
Oncotarget, 10(36), 3385-3399 (2019-06-06)
Metastatic and relapsed Ewing sarcoma typically afflicts the adolescent population and is largely fatal. These bone tumors are most commonly driven by the fusion oncoprotein EWS-FLI1. Ewing tumors demonstrate significant intra-tumoral heterogeneity, and individual tumor cells can express highly variable
LFA-1 and ICAM-1 expression induced during melanoma-endothelial cell co-culture favors the transendothelial migration of melanoma cell lines in vitro.
Ghislin, S; Obino, D; Middendorp, S; Boggetto, N; Alcaide-Loridan, C; Deshayes, F
BMC Cancer null
Mario C Manresa et al.
Gastroenterology, 159(5), 1778-1792 (2020-07-28)
Eosinophilic esophagitis (EoE) is an antigen-mediated eosinophilic disease of the esophagus that involves fibroblast activation and progression to fibrostenosis. Cytokines produced by T-helper type 2 cells and transforming growth factor beta 1 (TGFβ1) contribute to the development of EoE, but

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