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Anti-Cytokeratin 7 Antibody, clone OV-TL 12/30

clone OV-TL 12/30, Chemicon®, from mouse

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Anti-Anti-CD20, Anti-Anti-CK-20, Anti-Anti-CK20, Anti-Anti-K20, Anti-Anti-KRT21

biological source


Quality Level

antibody form

purified antibody

antibody product type

primary antibodies


OV-TL 12/30, monoclonal

species reactivity





flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable



NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification


Gene Information

human ... KRT7(3855)

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antibody form

purified antibody

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

antibody form

ascites fluid


OV-TL 12/30, monoclonal


M20, monoclonal


C-11, monoclonal


6B10, monoclonal

species reactivity


species reactivity

feline, human, rabbit, canine, bovine

species reactivity


species reactivity

rabbit, feline, hamster, goat, guinea pig, human, canine, sheep










flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable


immunohistochemistry (frozen sections): suitable, indirect immunofluorescence: 1:200 using frozen sections of human or animal tissue, western blot: suitable


immunocytochemistry: 15-30 μg/mL using methanol/acetone-fixed PTK-2 cells or A549 - human lung carcinoma cells, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 15-30 μg/mL using human skin, immunohistochemistry (frozen sections): suitable, microarray: suitable, western blot: suitable


immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunohistochemistry (frozen sections): suitable, indirect immunofluorescence: 1:300 using formalin-fixed, paraffin-embedded sections of human or animal tissue, microarray: suitable, western blot: suitable

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General description

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual cytokeratin polypeptides are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium.Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometry.


Cytokeratin 7. Reacts with specific glandular-type epithelia, and most carcinomas derived there from.


Cytoskeletal preparation of OTN-11 ovarian carcinoma cell line.


Anti-Cytokeratin 7 Antibody, clone OV-TL 12/30 detects level of Cytokeratin 7 & has been published & validated for use in FC, WB, IC, IH(P).

Immunohistochemistry on frozen tissue sections fixed with methanol. The antibody is also effective on paraffin embedded tissue sections that have been pretreated with 0.1% pronase/0.1% trypsin in PBS, 0.5% pepsin in 0.01N HCl or microwave pretreatment.

Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category


Replaces: MABT150

Physical form

Format: Purified
Liquid in PBS containing 0.09% sodium azide.

Storage and Stability

Maintain -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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