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MAB3554

Sigma-Aldrich

Anti-Cytokeratin 7 Antibody, clone OV-TL 12/30

clone OV-TL 12/30, Chemicon®, from mouse

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Synonym(s):
Anti-Anti-CD20, Anti-Anti-CK-20, Anti-Anti-CK20, Anti-Anti-K20, Anti-Anti-KRT21
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

OV-TL 12/30, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... KRT7(3855)

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This Item
C5301P2871C5176
antibody form

purified antibody

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

clone

OV-TL 12/30, monoclonal

clone

M20, monoclonal

clone

C-11, monoclonal

clone

6B10, monoclonal

species reactivity

human

species reactivity

feline, human, rabbit, canine, bovine

species reactivity

-

species reactivity

rabbit, feline, hamster, goat, guinea pig, human, canine, sheep

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

immunohistochemistry (frozen sections): suitable, indirect immunofluorescence: 1:200 using frozen sections of human or animal tissue, western blot: suitable

technique(s)

immunocytochemistry: 15-30 μg/mL using methanol/acetone-fixed PTK-2 cells or A549 - human lung carcinoma cells, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 15-30 μg/mL using human skin, immunohistochemistry (frozen sections): suitable, microarray: suitable, western blot: suitable

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunohistochemistry (frozen sections): suitable, indirect immunofluorescence: 1:300 using formalin-fixed, paraffin-embedded sections of human or animal tissue, microarray: suitable, western blot: suitable

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General description

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual cytokeratin polypeptides are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium.Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometry.

Specificity

Cytokeratin 7. Reacts with specific glandular-type epithelia, and most carcinomas derived there from.

Immunogen

Cytoskeletal preparation of OTN-11 ovarian carcinoma cell line.

Application

Anti-Cytokeratin 7 Antibody, clone OV-TL 12/30 detects level of Cytokeratin 7 & has been published & validated for use in FC, WB, IC, IH(P).
Immunoblotting

Immunohistochemistry on frozen tissue sections fixed with methanol. The antibody is also effective on paraffin embedded tissue sections that have been pretreated with 0.1% pronase/0.1% trypsin in PBS, 0.5% pepsin in 0.01N HCl or microwave pretreatment.

Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
Cytokeratins

Linkage

Replaces: MABT150

Physical form

Format: Purified
Liquid in PBS containing 0.09% sodium azide.

Storage and Stability

Maintain -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Ze Wu et al.
Cell communication and signaling : CCS, 20(1), 89-89 (2022-06-16)
Growth differentiation factor-11 (GDF-11), also known as bone morphogenetic protein-11, belongs to the transforming growth factor-beta superfamily. GDF-11 was first identified as an important regulator during embryonic development. Increasing evidence has demonstrated that GDF-11 regulates the development of various organs
Katerina Jirsova et al.
Investigative ophthalmology & visual science, 52(8), 5892-5898 (2011-06-23)
To present cytokeratin (CK)7 (OV-TL 12/30 clone) as a newly identified, reliable marker for distinguishing between the conjunctival and corneal surface epithelia, which will contribute to the precise diagnosis of limbal stem cell deficiency (LSCD). Corneal and conjunctival epithelial imprints
Hong-Jin Zhao et al.
Endocrinology, 159(7), 2815-2825 (2018-05-31)
Bone morphogenetic protein (BMP) 2 and activin A belong to the TGF-β superfamily and are highly expressed in human endometrium and placenta. Studies have demonstrated that activin A and BMP2 play essential roles in the process of early embryo implantation
Yuyang Tang et al.
Cell reports, 30(13), 4528-4539 (2020-04-03)
Radical cure of HIV-1 (HIV) is hampered by the establishment of HIV reservoirs and persistent infection in deep tissues despite suppressive antiretroviral therapy (ART). Here, we show that among HIV-positive women receiving suppressive ART, cells from placental tissues including trophoblasts
Jiali You et al.
Frontiers in cell and developmental biology, 9, 607332-607332 (2021-02-23)
Embryo implantation and trophoblast invasion are principal limiting factors of pregnancy establishment. Aberrant embryo development or improper trophoblast differentiation and invasion may lead to various unfavorable pregnancy-related outcomes, including early pregnancy loss (EPL). Our clinical data show that the serum

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