Merck
All Photos(1)

Documents

MABF415

Sigma-Aldrich

Anti-CD4 Antibody (mouse), clone GK1.5

clone GK1.5, 0.5 mg/mL, from rat

Sign Into View Organizational & Contract Pricing

Select a Size

Synonym(s):
T-cell surface glycoprotein CD4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, CD4
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

GK1.5, monoclonal

species reactivity

mouse

concentration

0.5 mg/mL

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG2bκ

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Cd4(12504)

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
MABF414MABF743MABF569
antibody form

affinity isolated antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

clone

GK1.5, monoclonal

clone

GK1.5, monoclonal

clone

GK1.5, monoclonal

clone

GK1.5, monoclonal

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

concentration

0.5 mg/mL

concentration

0.2 mg/mL

concentration

0.2 mg/mL

concentration

0.5 mg/mL

technique(s)

flow cytometry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable

technique(s)

flow cytometry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable

technique(s)

flow cytometry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable

technique(s)

flow cytometry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable

General description

The GK1.5 antibody reacts with mouse CD4, a 55 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The GK1.5 antibody is widely used as a phenotypic marker for CD4 expression. If used together, the GK1.5 antibody and an alternative antibody, Anti-Mouse CD4 clone RM4-5, will “compete” for binding, i.e. RM4-5 is able to block GK1.5 binding to cells. In contrast, the Anti-Mouse CD4 clone RM4-4 does not block binding of the GK1.5 antibody to cells (Arora S et al. 2006. Infect. Immun. 74: 4339-4348). The GK1.5 antibody is also reported to be cross-reactive with Syrian hamster CD4.

Specificity

M

Immunogen

Immunogen information for this antibody is proprietary information.

Application

Research Category
Inflammation & Immunology
This Rat Monoclonal Antibody Anti-CD4 (mouse), clone GK1.5, Cat. No. MABF415 is validated for use in Immunoprecipitation, Immunohistochemistry, Flow Cytometry for the detection of CD4.

Physical form

Format: Purified
Purified Rat Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.

Storage and Stability

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Nadia Duarte et al.
Journal of immunology (Baltimore, Md. : 1950), 173(5), 3112-3118 (2004-08-24)
A role for regulatory lymphocytes has been demonstrated in the pathogenesis of type 1 diabetes in the NOD mouse but the nature of these cells is debated. CD1d-restricted NKT lymphocytes have been implicated in this process. Previous reports of reduced
Martin Stenström et al.
European journal of immunology, 34(1), 56-65 (2004-02-19)
Natural killer T (NKT) lymphocytes rapidly produce several cytokines, including IL-4 and IFN-gamma, upon activation, and act as regulatory cells at an early interphase of innate and adaptive immune responses. They have been implicated as important elements in diverse immune
Shiki Takamura et al.
Journal of immunology (Baltimore, Md. : 1950), 175(4), 2541-2547 (2005-08-06)
CD4+ T cells play a crucial role in CTL generation in a DNA vaccination strategy. Several studies have demonstrated the requirement of CD4+ T cells for the induction of a sufficient immune response by coadministrating DNAs. In the present study
Fernanda A Lima et al.
Clinical and vaccine immunology : CVI, 19(9), 1382-1392 (2012-07-05)
Streptococcus pneumoniae is a pathogen of great importance worldwide. We have previously described the efficacy of a nasal vaccine composed of the pneumococcal surface protein A and the whole-cell pertussis vaccine as an adjuvant against a pneumococcal invasive challenge in
Keer Sun et al.
Journal of virology, 84(10), 5007-5014 (2010-03-05)
Interleukin-10 (IL-10) is an important anti-inflammatory molecule that can cause immunosuppression and long-term pathogen persistence during chronic infection of mice with viruses such as lymphocytic choriomeningitis virus. However, its specific role in immunity to acute viral infections is not fully

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service