Acetyl-CoA carboxylase 1 (UniProt: Q13085; also known as ACC1, ACC-alpha) is encoded by the ACACA (also known as ACAC, ACC1, ACCA) gene (Gene ID: 31) in human. It catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. ACC1 is a multi-subunit, biotin-dependent enzyme that catalyzes carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT). The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. Two isoforms fof ACC have been reported, alpha and beta that are encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. ACC1 levels are regulated under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. ACC1 activity is inhibited upon phosphorylation, which occurs when glucagon or epinephrine binds to the receptors or when the energy status of the cell is low, leading to the activation of the AMP-activated protein kinase. The presence of fatty acids inhibits the activities of ACC1. ACC is considered as an important target in the design of new anti-obesity drugs.
Clone 7H4.2 detects acetyl-CoA carboxylase 1 in human. It targets an epitope within 86 amino acids from the C-terminal end.
GST/His-tagged recombinant fragment corresponding to 86 amino acids from the C-terminal end of human Acetyl-CoA carboxylase 1.
Anti-Acetyl CoA Carboxylase 1 Antibody, clone 7H4.2, Cat. No. MABS830, is a mouse monoclonal antibody that targets Acetyl CoA Carboxylase 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Acetyl CoA Carboxylase 1 in human tonsil, human testis, and human cervical cancer tissue.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Acetyl CoA Carboxylase 1 in 10 µg of NIH/3T3 cell lyate.
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Acetyl CoA Carboxylase 1 in 10 µg of A431 cell lysate.
~265 kDa observed; 265.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Concentration: Please refer to lot specific datasheet.