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MABT411

Sigma-Aldrich

Anti-Desmocollin-2 Antibody, clone 7G6

clone 7G6, from mouse

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Synonym(s):
Desmocollin-2, Cadherin family member 2, Desmocollin-3, Desmosomal glycoprotein II, Desmosomal glycoprotein III
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

7G6, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... DSC3(1825)

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MABT335SAB1405728HPA012615
antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

clone

7G6, monoclonal

clone

5G11, monoclonal

clone

polyclonal

clone

polyclonal

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable (paraffin)

technique(s)

western blot: 1 μg/mL

technique(s)

immunohistochemistry: 1:200- 1:500

isotype

IgG1κ

isotype

IgG1κ

isotype

-

isotype

-

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General description

Desmocollin-2, also known as Desmocollin-3 or Desmosomal glycoprotein II, or Desmosomal glycoprotein III, and encoded by the gene DSC2/CDHF2/DSC3, is a component of intercellular desmosome junctions. Desmocollin-2 is involved in the interaction of plaque proteins and intermediate filaments that mediate cell-cell adhesion and interacts with DSP, PKP2 and JUP proteins. Additionally, Desmocollin-2 is important for epidermal cell positioning and stratification because it mediates the differential adhesiveness between cells that express different isoforms. Desmocollin-2 is localized to the cell membrane at cell junctions and desmosomes. Desmocollin-2 is expressed in epithelia, myocardium and lymph nodes. Defects in Desmocollin-2 may be associated with Familial arrhythmogenic right ventricular dysplasia 11 (ARVD11) a congenital heart disease characterized by infiltration of adipose and fibrous tissue into the right ventricle and loss of myocardial cells, resulting in ventricular and supraventricular arrhythmias.

Immunogen

Epitope: This antibody recognizes the extracellular domain of Desmocollin-2.
MBP-tagged recombinant protein corresponding to the extracellular domain of human Desmocollin-2.

Application

Immuonoprecipitation Anlaysis: A representative lot from an independent laboratory detected Desmocollin-2 in A431 cells transfected with C-terminal deletions of plakoglobin retaining 707 amino acids (Wahl, J. K. 3rd, et al. (1996). J Cell Sci. 109(Pt 5):1143-1154.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Desmocollin-2 in Primary human Keratinocytes (Franke, WW., et al (2006). Eur. J. Cell Biol. 85(2); 69-82.)

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Desmocollin-2 in A431 cells (Wahl, J. K. 3rd. (2005). J Cell Biochem. 96(2):390-403.).
Research Category
Cell Structure
Research Sub Category
ECM Proteins
This Anti-Desmocollin-2 Antibody, clone 7G6 is validated for use in western blotting, IP, IHC & ICC for the detection of Desmocollin-2.

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Desmocollin-2 in 10 µg of A431 cell lysate.

Target description

~110 kDa observed

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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