Merck
  • QIA113
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QIA113

Sigma-Aldrich

Caspase-8 Detection Kit (FITC-IETD-FMK)

All Photos(1)

usage

sufficient for 100 tests

Quality Level

100

species reactivity (predicted by homology)

mammals

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles
protect from light

technique(s)

flow cytometry: suitable

input

sample type intact cells

fluorescence

λex ~485 nm; λem ~535 nm

detection method

fluorometric

storage temp.

−20°C

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Caspase-8 Detection Kit (FITC-IETD-FMK)

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usage

sufficient for 100 tests

usage

sufficient for 1,000 multiwell tests, sufficient for 100 standard tests (2 ml)

usage

sufficient for 100 tests

usage

sufficient for 100 tests

species reactivity (predicted by homology)

mammals

species reactivity (predicted by homology)

-

species reactivity (predicted by homology)

-

species reactivity (predicted by homology)

mammals

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles, protect from light

storage condition

-

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

technique(s)

flow cytometry: suitable

technique(s)

-

technique(s)

flow cytometry: suitable

technique(s)

-

input

sample type intact cells

input

-

input

sample type cultured cells

input

sample type intact cells

General description

A fast, convenient, and sensitive method to measure caspase-8 in living cells. The fluorescent marker, FITC-IETD-FMK, is a labeled, cell-permeable, non-toxic inhibitor that binds irreversibly to activated caspase-8 in living cells. Caspase-8 can be detected by the measurement of fluorescence in a fluorescent microplate reader, flow cytometer, or by fluorescence microscopy.

Specificity

A broad range of species

Components

Labeled Caspase-8 Inhibitor (FITC-IETD-FMK), Wash Buffer, Unlabeled Caspase Inhibitor (Z-VAD-FMK), and a user protocol.

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Specifications

Assay Time: 1.5 h

Storage and Stability

Upon arrival store the entire contents of the kit at -20°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

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