Merck
All Photos(1)

S4117

Millipore

Formamide, Deionized

Synonym(s):
DI formamide, formamide for hybridization
eCl@ss:
32160405

packaging

pkg of 500 mL

Quality Level

manufacturer/tradename

Chemicon®

technique(s)

hybridization: suitable

shipped in

wet ice

General description

This product is intended for use in hybridization applications for in situ, Southern, Northern, dot and slot blot analyses, and colony, plaque, and YAC screening. Formamide is used primarily as a component in probe hybridization solutions to promote probe hybridization specificity. It denatures nucleic acids by disrupting hydrogen bonds.

Application

Dilute formamide according to specific application requirements. Formamide concentrations of 45-50% are typically used in Southern and Northern analyses and a 50% or greater concentration for in situ hybridization techniques. In DNA to DNA hybridization, each 1% increase in formamide concentration lowers the Tm by 0.7%.
Research Category
All

Quality

Each lot of material is pre-filtered through a mixed-ion exchange resin to eliminate products of decomposition and residual metal cations. This filtering process guarantees that each lot has a final purity of greater than 99.5% and an adsorbance value of less than 0.05 adsorbance units at 280nm. Each lot then undergoes rigid quality control testing to insure that assay specifications are met prior to release.

Physical form

This preperation of formamide is deionized, triple distilled and packaged under nitrogen and is free of detectable DNAse, RNAse, phosphatase, and protease activity.

Storage and Stability

Store at -20°C. Keep cap tightly closed after opening or aliquot and store at -15°C to -25°C.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Carc. 2 - Repr. 1B - STOT RE 2 Oral

Target Organs

Blood

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flash Point(F)

305.6 °F

Flash Point(C)

152 °C


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH SVHC Candidate List

CAS No.

EU REACH Annex XVII (Restriction List)

CAS No.

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

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