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SCT100

Sigma-Aldrich

BioTracker NucView® 488 Green Caspase-3 Dye (DMSO)

Live cell imaging apoptosis dye for caspase-3/7 enzyme activity used to detect programmed cell death using fluorescence microscopy or flow cytometry applications.

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Synonym(s):
Live cell caspase-3 apoptosis probe, Live cell imaging probe

technique(s)

cell based assay: suitable

detection method

fluorometric

shipped in

ambient

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SCT101SCT102SCT104
shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

detection method

fluorometric

detection method

fluorometric

detection method

fluorometric

detection method

fluorometric

General description

Caspases are a family of protease enzymes that play essential roles in programmed cell death, termed apoptosis. Caspase-3 is a caspase protein that interacts with caspase-8 and caspase-9 and sequential activation of plays a central role in the execution-phase of cellular apoptosis.

The BioTracker NucView 488 Green Caspase-3 Dye provides a convenient live cell tool for profiling apoptotic cell populations based on caspase-3/7 activity using either fluorescence microscopy or flow cytometry. In contrast to other fluorogenic caspase substrates or fluorescent caspase inhibitor based (FLICA) assays, the BioTracker NucView 488 Caspase-3 Dye can be used to detect caspase-3/7 activity within individual intact cells without inhibiting apoptosis progression. The substrate consists of a fluorogenic DNA dye coupled to the caspase-3/7 DEVD recognition sequence. The substrate, which is initially non-fluorescent, penetrates the plasma membrane and enters the cytoplasm. In apoptotic cells, caspase-3/7 cleaves the substrate, releasing the high-affinity DNA dye, which migrates to the cell nucleus and stains DNA with bright green fluorescence. Thus, NucView 488 Caspase-3 Substrate is bifunctional, allowing detection of caspase-3/7 activity and visualization of morphological changes in the nucleus during apoptosis. The dye is formaldehyde-fixable and compatible with subsequent immunostaining. The dye is offered in DMSO and PBS (phosphate-buffered saline) formulations. The substrate in PBS is formulated for use in cells that are sensitive to DMSO toxicity.

Spectral Properties
Absorbance: 500nm
Emission: 530nm

Application

Live cell fluorescent imaging
Live cell imaging apoptosis dye for caspase-3/7 enzyme activity used to detect programmed cell death using fluorescence microscopy or flow cytometry applications.
Research Category
Cell Imaging
Research Sub Category
Live Cell Dye

Quality

Spectral Properties
Absorbance: 500nm
Emission: 530nm

Physical form

Liquid

Storage and Stability

Store BioTracker NucView 488 Green Caspase-3 Dye, 1 mM in DMSO at 2-8ºC. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

Legal Information

NucView is a registered trademark of Biotium Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

188.6 °F - (refers to pure substance)

Flash Point(C)

87 °C - (refers to pure substance)


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