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Sigma-Aldrich

ATP Cell Viability Luciferase Assay

A highly sensitive firefly luciferase cell based assay for quantifying ATP in cell cultures used to measure cell viability.

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Synonym(s):
Luminescent Cell Viability Assay, Cell Toxcity Assay, Bioluminescent Cell Viability Assay

technique(s)

cell based assay: suitable

detection method

chemiluminescent

shipped in

ambient

Related Categories

General description

Because ATP is an indicator of metabolically active cells, the number of viable cells can be assessed based on the amount of ATP available. The ATP Cell Viability Luciferase Assay offers a highly sensitive homogenous assay for quantifying ATP in cell cultures. This kit takes advantage of Firefly luciferase’s use of ATP to oxidize D-Luciferin and the resulting production of light in order to assess the amount of ATP available in cell cultures. The sensitive assay procedure involves a single addition of ATP detection cocktail directly to cells cultured in a serum-supplemented medium. No cell washing, medium removal and multiple pipetting are required. The kit can be used to detect as little as a single cell or 0.01 picomoles of ATP. The signal produced is linear within 6 orders of magnitude. By relating the amount of ATP to the number of viable cells, the assay has wide applications, ranging from the determination of viable cell numbers to cell proliferation to cell cytotoxicity.

The ATP Cell Viability Luciferase Assay is a flash-type luminescence assay designed for individual sample detection by using a luminometer in a single sample format or a luminometer with an injector in 96-well plate format. The luminescence signal generated is stable for about 1 minute.

Application

A highly sensitive firefly luciferase cell based assay for quantifying ATP in cell cultures used to measure cell viability.
Cell Viability Assay
Research Category
Cell Signaling
Research Sub Category
Live Cell Dye

Components

1) D-Luciferin (CS224519): 4 X 10 mg

2) Firefly Luciferase (CS224520): 5 X 200 µ

3) ATP Assay Buffer (CS224521): 1 X 10 mL

4) ATP, 2µM (CS224522): 1 X 100 µL

Physical form

Liquid

Storage and Stability

Store ATP Cell Viability Luciferase Assay at -80°C. Avoid repeated freeze-thaw cycles. Product is stable for at least 6 months from date of receipt when stored as recommended.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3


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Articles

Firefly luciferase is a sensitive reporter for gene studies due to its absence in mammalian cells or tissues.

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