ST1200

Anti-Ubiquitin Mouse mAb (FK2)

liquid, clone FK2, Calbiochem^®

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: unpurified
• antibody product type: primary antibodies
• clone: FK2, monoclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity (predicted by homology): all
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• isotype: IgG1
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with myeloma cells. Recognizes K²⁹-, K⁴⁸-, and K⁶³-linked polyubiquitinylated and monoubiquitinylated proteins.

Recognizes K²⁹-, K⁴⁸-, and K⁶³-linked polyubiquitinylated and monoubiquitinylated proteins, but not free ubiquitin, in Raji cells.

This Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the detection of Ubiquitin.

Immunogen

human

unpurified polyubiquitinylated lysozyme

Packaging

100 μg in Glass bottle

Please refer to vial label for lot-specific concentration.

Application

ELISA (see application references)
Immunoblotting (1:1000)
Immunoprecipitation (see application references and comments)
Immunofluorescence (see application references)

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS.

Preparation Note

Raji cells

Reconstitution

Following initial thaw, aliquot and freeze (-20°C). Store diluted antibody at 4°C for up to 1 month.

Other Notes

Does not recognize free ubiquitin. For immunoprecipitation, once the antibody is immobilized, it demonstrates affinity for free ubiquitin as well as for poly- and monoubiquitinylated proteins. Antibody should be titrated for optimal results in individual systems.

Matsumoto, M., et al. 2005. Proteomics 5, 4545.
Boname, J.N., et al. 2001. Immunity 15, 627.
Campbell, V., et al. 2001. Neuron 32, 1013.
Fujimuro, M., et al. 2003. FEBS Letts. 349, 173.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable