UFC9010

Amicon^® Ultra-15 Centrifugal Filter Unit

Ultracel-10 regenerated cellulose membrane, 15 mL sample volume

• Synonym(s): 10 kDa MWCO, 15 mL sample volume, Centrifugal concentrator
• eCl@ss: 36100101
• NACRES: NB.24

Properties

• material: polyethylene cap; polyethylene liner; polypropylene tube; regenerated cellulose membrane; regenerated cellulose support; styrene-butadiene housing
• Quality Level: 400
• sterility: non-sterile
• product line: Amicon^®
• manufacturer/tradename: Amicon^® Ultra
• parameter: 15 mL sample volume
• technique(s): protein extraction: suitable; protein purification: suitable
• L: 12.1 cm (4.807 in.)
• diam.: 2.97 cm
• filtration area: 7.6 cm²
• volume: 15 mL
• pore size: 10 kDa NMWCO
• shipped in: ambient

Description

General description

Amicon^® Ultra 15 mL centrifugal devices with Ultracel^® 10,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, Cytochrome C (12.4 kDa) at 0.25 mg/ml initial concentration is retained at >95% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.

The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon^® Ultra 15 mL filters deliver the highest recovery with short spin times.

Application

• Ultrafiltration with 10,000 Dalton molecular weight cutoff Ultracel^® membrane.
• Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
• Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS.
• Concentration of dilute or pre-purified proteins from column eluents.
• Desalting, buffer exchange and protein dialysis.

Features and Benefits

• Ultracel^® regenerated cellulose membrane with 10 kDa NMWCO delivers retentate recovery of greater than 90%.
• Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10-15 minutes).
• Heat-sealed membrane minimizes downstream extractables.
• 100% integrity tested for reliable performance.
• Convenient sample monitoring with translucent housing and volume gradations.
• Direct pipettor sample access eliminates processing step to recover concentrate.
• High concentration factors of 80–100X.

Physical form

Color Code: Clear with pink cap

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany

ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany