11418165001

Biotin Protein Labeling Kit

sufficient for 5 labeling reactions, kit of 1 (5 components), suitable for immunoblotting, suitable for ELISA

• Synonym(s): Biotin, protein labeling

Properties

• usage: sufficient for 5 labeling reactions
• Quality Level: 100
• packaging: kit of 1 (5 components)
• manufacturer/tradename: Roche
• technique(s): ELISA: suitable; immunoblotting: suitable
• storage temp.: 2-8°C

Description

General description

For labeling of proteins with biotin.
Free amino acids of the protein to be labeled react with D-biotinoyl-ε-aminocaproic acid-N-hydroxy-succinimide ester (biotin-7-NHS) by forming a stable amino bond. Non reacted biotin-7-NHS is separated on a Sephadex G-25 column.

Application

Biotin protein labeling kit has been used to produce biotinylated denatured bovine serum albumin (d-BSA) for coating cadmium telluride (CdTe) quantum dot (QD) conjugates.
The kit is suitable for the labeling of proteins with biotin for immunochemistry, immunoblotting, and ELISA.

Packaging

1 kit containing 5 components.

Quality

Function tested.

Preparation Note

Working solution: Preparation of the solutions

• Blocking solution: Dissolve the contents of the bottle blocking reagent (green screw cap) in 300 ml double-distilled water.
• PBS solution: Dissolve the contents of the bottle PBS (blue screw cap) in 1 I double-distilled water.
• Biotin-7-NHS solution: The solution should be freshly prepared before labeling. Add to the contents of the reaction vial which includes biotin-7-NHS 250 μI DMSO and vortex the solution several times. The resulting concentration of the biotin-7-NHS solution is 20 mg/ml. For the labeling mix, the volume of the biotin-7-NHS solution should not exceed 5% of the total reaction volume (e.g., add maximal 50 μl biotin-7-NHS solution to 1 ml protein solution).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

• Blocking Reagent (green screw cap)
• Phosphate Buffered Saline (PBS) (blue screw cap)
• D-Biotinoyl-ε-aminocaproic acid-N-hydroxysuccinimide ester (Biotin-7-NHS)
• Dimethylsulfoxide (DMSO) (red screw cap)
• Sephadex G-25 Columns

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1