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Millipore

Urea Broth

suitable for microbiology, NutriSelect® Basic

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UNSPSC Code:
41171606
NACRES:
NA.74

sterility

non-sterile

Quality Level

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

composition

dipotassium hydrogen phosphate, 9.5 g/L
phenol red, 0.01 g/L
potassium dihydrogen phosphate, 9.1 g/L
urea, 20 g/L
yeast extract, 0.1 g/L

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbe id | specific enzyme detection: suitable
microbiological culture: suitable

final pH

6.8±0.2 (25 °C)

application(s)

clinical testing
environmental
food and beverages

microbiology

suitability

nonselective and identification for Enterobacter spp.
nonselective and identification for Escherichia coli
nonselective and identification for Klebsiella spp.
nonselective and identification for Proteus spp.
nonselective and identification for Salmonella spp.
nonselective and identification for coliforms
nonselective and identification for enterobacteriaceae

Application

Differential medium for the detection of urea-utilizing microorganisms, acc. to Rustigian and Stuart (1941).

Preparation Note

Dissolve 38.7 g in 1 litre distilled water and filter sterilize. This medium must NOT be heated.

Other Notes

Adaption of urea broth for radiosterilization

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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E Eisenberg et al.
The Journal of applied bacteriology, 58(1), 21-25 (1985-01-01)
Urea broth, a medium used for the identification of the genus Proteus, was sterilized by gamma radiation, using radiation doses of 1-1.5 Mrad. The radiation-sterilized medium, modified by adding sodium ascorbate and increasing its phenol red and yeast extract content
R. Rustigian et al.
Proceedings of the Society For Experimental Biology and Medicine. Society For Experimental Biology and Medicine, 47, 108-108 (1941)
W.W. Feguson et al.
The Journal of Laboratory and Clinical Medicine, 28, 1715-1715 (1943)

Articles

Salmonella, with 2,300+ serotypes, causes half of food-borne illnesses, often from dairy, poultry, and eggs.

An article concerning selective growth media for differentiation and detection of Escherichia coli and other coliforms.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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